Vinblastine, while not causing depolymerization of spindle microtubules, effectively blocks mitosis (e.g., IC50 0.8 nM in HeLa cells), leading to apoptotic cell death [2]. In NB4 cells, vinblastine induces alterations in p53 and DNA fragmentation. Its antiproliferative effect involves inducing apoptosis by disrupting the Bax/Bcl-2 balance. Vinblastine also suppresses NFκB expression and activity while activating JNK, resulting in apoptosis through a caspase-dependent pathway [3]. Vinblastine induces apoptosis, characterized by mitochondrial membrane potential loss, cytochrome c and apoptosis-inducing factor release, caspase-9 and caspase-3 activation, and Poly (ADP-ribose) Polymerase cleavage [4].
体内研究
Vinblastine, an extensively utilized anticancer medication, is associated with undesirable side effects. Its conjugation with carrier molecules presents a potentially effective strategy for mitigating these adverse effects [5].
体外研究
Vinblastine, while not causing depolymerization of spindle microtubules, effectively blocks mitosis (e.g., IC50 0.8 nM in HeLa cells), leading to apoptotic cell death [2].
In NB4 cells, vinblastine induces alterations in p53 and DNA fragmentation. Its antiproliferative effect involves inducing apoptosis by disrupting the Bax/Bcl-2 balance. Vinblastine also suppresses NFκB expression and activity while activating JNK, resulting in apoptosis through a caspase-dependent pathway [3].
Vinblastine induces apoptosis, characterized by mitochondrial membrane potential loss, cytochrome c and apoptosis-inducing factor release, caspase-9 and caspase-3 activation, and Poly (ADP-ribose) Polymerase cleavage [4].
Vinblastine sulfate 细胞研究
细胞系
浓度
检测类型
检测时间
活动说明
数据源
142BR cells
Cytotoxicity assay
48 h
Cytotoxicity against human 142BR cells after 48 hrs by SRB assay, IC50=37.6 μM
19467877
A375 cells
Cytotoxicity assay
48 h
Cytotoxicity against human A375 cells after 48 hrs by SRB assay, IC50=7.2 μM
19467877
A549 cells
Proliferation assay
48 h
Antiproliferative activity against human A549 cells after 48 hrs by MTS assay, IC50=2.36 μM
22546674
ACHN cells
Cytotoxicity assay
48 h
Cytotoxicity against human ACHN cells after 48 hrs by SRB assay, IC50=22.7 μM
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