Ofloxacin (Hoe-280) at a dosage of 20 mg/kg, norfloxacin at 40 mg/kg, pefloxacin mesylate dihydrate at 40 mg/kg, and ciprofloxacin at 50 mg/kg are administered by gavage to test animals twice daily for three consecutive weeks. Six weeks post-treatment, the animals are euthanized, and Achilles tendon specimens are harvested for biomechanical testing using a computer-monitored tensile testing machine^[3].

Ofloxacin (Hoe-280) is a fluoroquinolone antibiotic primarily acting through the inhibition of bacterial DNA gyrase. It exhibits a broad spectrum of activity against aerobic Gram-negative and Gram-positive bacteria in vitro, but shows limited efficacy against anaerobes^[1].

Unlike many other front-line antibacterial drugs that target cell wall or protein synthesis, ofloxacin and other 4-quinolones uniquely interfere with bacterial DNA synthesis, distinguishing their mechanism of action in treating bacterial infections^[2].