Amantadine, known chemically as 1-Adamantanamine, is a potent orally active antiviral agent effective against influenza A viruses. It also blocks various ion channels, including NMDA and M2, and inhibits Coronavirus ion channels, showcasing its broad antiviral capabilities. Additionally, Amantadine possesses anti-orthopoxvirus and anticancer activities, making it applicable in Parkinson's disease, postoperative cognitive dysfunction (POCD), and COVID-19 research[1].[2].[3].[4].[5].[6].
体内研究
In animal models, Amantadine at a dosage of 25 mg/kg, administered intraperitoneally once daily for three days, effectively reduces surgery-induced neuroinflammation and mitigates associated impairments in learning and memory[5].
体外研究
Research has shown that Amantadine (0-500 µM, 26 h) impedes SARS-CoV-2 replication, demonstrating IC50 concentrations ranging between 83 and 119 µM[4].
In cell studies, Amantadine significantly curtails the proliferation of HepG2 and SMMC‑7721 cells over concentrations ranging from 0 to 100 µg/mL over periods of 24 to 72 hours[6].
Amantadine also impacts cellular growth by arresting the cell cycle in the G0/G1 phase and promoting apoptosis when applied at concentrations from 0 to 75 µg/mL over 48 hours[6].
Furthermore, at similar concentrations and duration, Amantadine downregulates cell cycle‑related genes and proteins such as cyclin D1, cyclin E, and CDK2, diminishes Bcl-2 levels, and elevates Bax protein and mRNA levels, influencing apoptotic pathways[6].
搜索
