Nocodazole | Oncodazole | Microtubule Inhibitor | AmBeed-信号通路专用抑制剂

首页 / / / / Nocodazole

诺考达唑 /Nocodazole {[allProObj[0].p_purity_real_show]}

货号：A260772 同义名： Oncodazole;R17934

Nocodazole 是一种微管抑制剂，对微管具有高亲和力。Nocodazole 主要用于研究细胞分裂、微管动态和细胞周期调控。

Nocodazole 化学结构
CAS号：31430-18-9

Nocodazole 3D分子结构
CAS号：31430-18-9

规格	价格	会员价	库存	数量
{[ item.pr_size ]}	{[ getRatePrice(item.pr_rmb, 1,1) ]} {[ getRatePrice(item.pr_rmb_sale, 1,1) ]} {[ suihuo_tips(item.pr_tag_price) ]}	{[ getRatePrice(item.pr_rmb, 1,1) ]} {[ getRatePrice(item.pr_rmb,item.pr_rate,1) ]} {[ suihuo_tips(item.pr_tag_price) ]}	{[ getRatePrice(item.pr_rmb, 1,1) ]}{[ suihuo_tips(item.pr_tag_price) ]}	{[ getRatePrice(item.pr_rmb_sale, 1,1) ]} {[ getRatePrice(item.pr_rmb,item.pr_rate,item.mem_rate) ]} {[ getRatePrice(item.pr_rmb,1,item.mem_rate) ]}	现货	1周咨询	- +

购物车0 收藏询单

sales@ambeed.cn tech@ambeed.cn 400-920-2911 产品手册产品订购技术咨询
快速发货顺丰冷链运输，1-2 天到达

品质保证

技术支持

免费溶解

Nocodazole 纯度/质量文件产品仅供科研

货号：A260772 标准纯度： {[allProObj[0].p_purity_real_show]} 产品说明书

批次查询：批次纯度: COA SDS NMR HPLC CNMR LC-MS

全球学术期刊中引用的产品: • ACS Appl. Nano Mater., 2025. Ambeed. [ A188026 ]; • Eur. J. Clin. Microbiol. Infect. Dis., 2025. Ambeed. [ A151161 ]; • JJC, 2025, 20(Special Issue)): 27-39. Ambeed. [ A713856 ]; • bioRxiv, 2025. Ambeed. [ A538667 , A714873 , A126729 , A126049 , A446054 ]; • Nature Communications, 2025, 16, 1871. Ambeed. [ A508148 ]; 更多 >

自噬亚型比较
转化生长因子-β 亚型比较
Bcr-Abl 亚型比较

产品名称	Autophagy ↓ ↑	其他靶点	纯度
SBI-0206965	+++ ULK1, IC50: 108 nM ULK2, IC50: 711 nM		95%
Hydroxychloroquine sulfate	✔		99%
Valproic acid sodium	✔	HDAC	97%
PFK-015	++ PFKFB3, IC50: 207 nM		99%+
MRT68921 hydrochloride	++++ ULK1, IC50: 2.9 nM ULK2, IC50: 1.1 nM		99%+
ROC-325	✔		99%+
Autophinib	+++ Autophagy, IC50: 40 nM		99%
Lys05	✔		99%+
1. 鼠标悬停在“+”上可以显示相关IC50的具体数值。"+"越多，抑制作用越强。2. "✔"表示该化合物对相应的亚型有抑制作用，但抑制强度暂时没有相关数据。

产品名称	ALK1 ↓ ↑	ALK2 ↓ ↑	ALK3 ↓ ↑	ALK4 ↓ ↑	ALK6 ↓ ↑	Smad3 ↓ ↑	TGF-β ↓ ↑	TGFβRI/ALK5 ↓ ↑	TGFβRII ↓ ↑	纯度
LDN193189	++++ ALK1, IC50: 0.8 nM	++++ ALK2, IC50: 0.8 nM	+++ ALK3, IC50: 5.3 nM		+++ ALK6, IC50: 16.7 nM					99%+
LDN-212854	++++ ALK1, IC50: 2.4 nM	++++ ALK2, IC50: 1.3 nM	+ ALK3, IC50: 85.8 nM	+ ALK4, IC50: 2133 nM				+ ALK5, IC50: 9276 nM		99%+
ML347	++ ALK1, IC50: 46 nM	++ ALK2, IC50: 32 nM								98%
K02288	++++ ALK1, IC50: 1.8 nM	++++ ALK2, IC50: 1.1 nM	++ ALK3, IC50: 34.4 nM		+++ ALK6, IC50: 6.4 nM					99%+
LDN-193189 dihydrochloride	++++ ALK1, IC50: 0.8 nM	++++ ALK2, IC50: 0.8 nM	+++ ALK3, IC50: 5.3 nM		+++ ALK6, IC50: 16.7 nM					99%
LDN-214117		++ ALK2, IC50: 24 nM								98%
DMH-1		+ ALK2, IC50: 107.9 nM								99%+
SB-505124				+ ALK4, IC50: 129 nM				++ ALK5, IC50: 47 nM		99%+
Vactosertib				+++ ALK4, IC50: 13 nM				+++ ALK5, IC50: 11 nM		99%+
Alantolactone						✔				98%
SIS3						✔				97%
Pirfenidone							✔			98%
Hesperetin							✔			97%
RepSox								++++ TGFβR1(ALK5), IC50: 4 nM		98%
GW788388								+++ ALK5, IC50: 18 nM		98%
LY364947								++ TGFβRI, IC50: 59 nM	+ TGFβRII, IC50: 0.4 μM	98%
SD-208								++ TGF-βRI (ALK5), IC50: 48 nM		99%
SB-525334								+++ TGFβR1(ALK5), IC50: 14.3 nM		99%+
LY2109761								++ TβRI, Ki: 38 nM	+ TβRII, Ki: 300 nM	99%+
Galunisertib								++ TβRI, IC50: 56 nM		98%
SB 431542								+ ALK5, IC50: 94 nM		99%+
1. 鼠标悬停在“+”上可以显示相关IC50的具体数值。"+"越多，抑制作用越强。2. "✔"表示该化合物对相应的亚型有抑制作用，但抑制强度暂时没有相关数据。

产品名称	Abl ↓ ↑	Bcr-Abl ↓ ↑	其他靶点	纯度
NVP-BHG 712	+ c-Abl, IC50: 1.667 μM			99%+
KW-2449	+++ Abl (T315I), IC50: 4 nM Abl, IC50: 14 nM		FLT3	99%+
Ponatinib	++++ Abl, IC50: 0.37 nM			98%
AT9283				99%+
Imatinib Mesylate	+ v-Abl, IC50: 600 nM		PDGFR,c-Kit	99%
Danusertib	++ Abl, IC50: 25 nM		RET	99%+
Rebastinib	++++ u-Abl1 (T315I), IC50: 5 nM p-Abl1 (native), IC50: 0.75 nM		Tie-2,FLT3	99%+
PP121	++ Abl, IC50: 18 nM		VEGFR,PDGFR	99%+
GNF-7	+++ M351T, IC50: 133 nM E255V, IC50: 122 nM			99%+
Olverembatinib dimesylate	++++ Abl (G250E), IC50: 0.35 nM Abl, IC50: 0.34 nM			99%
Dasatinib monohydrate	++++ Abl , IC50: 0.6 nM		Src	98%
Dasatinib	++++ Abl, IC50: 0.6 nM		Src	98%
Bafetinib	+++ Abl, IC50: 5.8 nM			98+%
GNF-2		+ Bcr-Abl (K562 cell line), IC50: 273 nM Bcr-Abl (SUP-B15 cell line), IC50: 268 nM		98%+
Degrasyn		+ Bcr-Abl, IC50: 1.8 μM	DUB	98+%
GNF-5		++ Bcr-Abl, IC50: 220 nM		99%
Radotinib		++ BCR-ABL1, IC50: 34 nM		98+%
PD173955			Src	99%+
Nilotinib		++ Bcr-Abl, IC50: <30 nM		98%
1. 鼠标悬停在“+”上可以显示相关IC50的具体数值。"+"越多，抑制作用越强。2. "✔"表示该化合物对相应的亚型有抑制作用，但抑制强度暂时没有相关数据。

Nocodazole 生物活性

描述

Microtubules, part of the cytoskeleton that provides structure and shape to the cytoplasm of cells, are tubular polymers of tubulin formed by polymerization. Nocodazole is a standard anti-microtubule agent that interferes with the polymerization and now be mostly used as depolymerizing drug in basic cell biology research^[8]. Nocodazole can be used as a high-affinity ligand for the cancer-related kinases including Abl phosphorylated, c-Kit, BRAF, and MEK. Furthermore, Nocodazole induces apoptosis in chronic lymphocytic leukemia cells, inhibits insulin-stimulated glucose transport and decreases apoptosis in some human colon carcinoma cells^[9]. In vitro Tubulin Polymerization Assays, Nocodazole inhibited tumor cell lines including NCIH460, SKOV3, BT549,451LU, COLO-205 at 48h with IC50 values 68nM, 80nM, 69nM, 72nM, 88nM respectively, which was reflected by a decreased level of steady state turbidity in a dose-dependent manner^[5]. To learn how the spindle assembly checkpoint (SAC) inhibitory is extinguished, 9A-Hec1-expressing cells exhibited a robust mitotic arrest after incubation in 5 mM nocodazole for 5 h. As comparison, WT-Hec1 was treated with a moderately low dose (300 nM) of nocodazole, which resulted in the loss of all non-kinetochore spindle microtubules but retention of kinetochore–microtubule^[6]. In addition, nocodazole can also improve the CRISPR-mediated HDR efficiency, a 600-bp homology in both arms leads to high-level genome knock in, with 97–100% of the donor insertion events being mediated by HDR. The combined use of CCND1, a cyclin that functions in G1/S transition, and nocodazole, a G2/M phase synchronizer, doubles HDR efficiency to up to 30% in iPSCs, the results showed an additive effect on enhancing precise genome editing^[7].

作用机制

Nocodazole will bind to β-Tb and block polymerization, arrest mammalian cells in mitosis and release them into cytokinesis and G1 as well as mis-regulate mitotic kinases^[8].

Nocodazole 细胞研究

细胞系	浓度	检测类型	检测时间	活性说明	数据源
human 451LU cells		Proliferation assay	48 h	Antiproliferative activity against human 451LU cells after 48 hrs by alamar blue assay, IC50=72 nM	21563750
human A549 cells		Proliferation assay	48 h	Antiproliferative activity against human A549 cells after 48 hrs by SRB assay, GI50=20 nM	25827522
human A549 cells		Cytotoxic assay	24 h	Cytotoxicity against human A549 cells after 24 hrs by MTT assay, IC50=36.6 nM	20110137
human A549 cells		Growth inhibition assay		Growth inhibition of human A549 cells by Sulforhodamine B assay, GI50=0.1 μM	21106458

Nocodazole 动物研究

Dose	Mice: 15 mg/kg - 60 mg/kg^[3] (i.p.), 5 mg/kg^[4] (i.p.)
Administration	i.p.

Nocodazole 参考文献

[1]7(1):53-6. doi: 10.1002/cmdc.201100410. Epub 2011 Oct 14.

[2]7(1):53-6. doi: 10.1002/cmdc.201100410. Epub 2011 Oct 14.

[3]54(12):4247-63. doi: 10.1021/jm200436t. Epub 2011 May 26.

[4]6:10036. doi: 10.1038/ncomms10036

[5]Prinz H. N-benzoylated phenoxazines and phenothiazines: synthesis, antiproliferative activity, and inhibition of tubulin polymerization. J Med Chem. 2011 Jun 23;54(12):4247-63. doi: 10.1021/jm200436t. Epub 2011 May 26.

[6]Tauchman EC. Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells. Nat Commun. 2015 Dec 1;6:10036. doi: 10.1038/ncomms10036

[7]Zhang JP. Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage. Genome Biol. 2017 Feb 20;18(1):35. doi: 10.1186/s13059-017-1164-8.

Nocodazole 实验方案

计算器

存储液制备

1mg

5mg

10mg

1 mM

5 mM

10 mM

3.32mL

0.66mL

0.33mL

16.59mL

3.32mL

1.66mL

33.19mL

6.64mL

3.32mL

Nocodazole 技术信息

CAS号	31430-18-9
分子式	C₁₄H₁₁N₃O₃S
分子量	301.32

别名	Oncodazole;R17934;NSC238159
运输	蓝冰

存储条件

In solvent -20°C:3-6个月-80°C:12个月

Pure form Sealed in dry,Store in freezer, under -20°C

溶解方案

细胞实验：

DMSO: 18 mg/mL(59.74 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

动物实验配方

IP 2% DMSO+2% Tween80+30% PEG300+water 0.4 mg/mL clear

PO 0.5% CMC-Na 65 mg/mL suspension

细胞研究

细胞系	浓度	检测类型	检测时间	活性说明	数据源

human 451LU cells		Proliferation assay	48 h	Antiproliferative activity against human 451LU cells after 48 hrs by alamar blue assay, IC50=72 nM	21563750
human A549 cells		Proliferation assay	48 h	Antiproliferative activity against human A549 cells after 48 hrs by SRB assay, GI50=20 nM	25827522
human A549 cells		Cytotoxic assay	24 h	Cytotoxicity against human A549 cells after 24 hrs by MTT assay, IC50=36.6 nM	20110137
human A549 cells		Growth inhibition assay		Growth inhibition of human A549 cells by Sulforhodamine B assay, GI50=0.1 μM	21106458
human BT549 cells		Proliferation assay	48 h	Antiproliferative activity against human BT549 cells after 48 hrs by alamar blue assay, IC50=69 nM	21563750
human COLO205 cells		Proliferation assay	48 h	Antiproliferative activity against human COLO205 cells after 48 hrs by alamar blue assay , IC50=88 nM	21563750
human HaCaT cells		Cytotoxic assay	48 h	Cytotoxic activity against human HaCaT cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay, IC50=8.9 μM	26231080
human HeLa cells		Proliferation assay	48 h	Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay, GI50=15 nM	25827522
human Jurkat T cells		Function assay	36 h	Induction of microtubule damage in human Jurkat T cells overexpressing Bcl2 after 36 hrs by immunostaining method	22197393
human KB/HeLa cells		Function assay	24 h	Cell cycle arrest in human KB/HeLa cells assessed as G2/M phase accumulation after 24 hrs by FACS analysis, EC50=91 nM	19220018
human MCF7 cells		Growth inhibition assay		Growth inhibition of human MCF7 cells by Sulforhodamine B assay, GI50=0.1 μM	21106458
human MDA-MB-231 cells		Proliferation assay	48 h	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by SRB assay, GI50=17 nM	25827522
human MIAPaCa2 cells		Proliferation assay	48 h	Antiproliferative activity against human MIAPaCa2 cells assessed as growth inhibition after 48 hrs by SRB assay, GI50=0.95 μM	25131956
human NCI-H460 cells		Proliferation assay	48 h	Antiproliferative activity against human NCI-H460 cells after 48 hrs by alamar blue assay, IC50=68 nM	21563750
human NCI-H460 cells		Proliferation assay	48 h	Antiproliferative activity against human NCI-H460 cells after 48 hrs by XTT assay, IC50=0.15 μM	20537765
human PANC1 cells		Proliferation assay	48 h	Antiproliferative activity against human PANC1 cells after 48 hrs by SRB assay, GI50=10 nM	25827522
human PC3M cells	0.5 ug/ml	Function assay	24 h	Induction of apoptosis in human PC3M cells assessed as accumulation at subG1 phase at 0.5 ug/ml after 24 hrs by flow cytometry	18247573
human RKO cells		Proliferation assay		Antiproliferative activity against human RKO cells by XTT assay, IC50=0.11 μM	19220018
human SF268 cells		Cytotoxic assay	48 h	Cytotoxicity against human SF268 cells after 48 hrs by XTT assay, IC50=0.3 μM	21705223
human SGC7901 cells		Function assay	48 h	Induction of distortions in tubulin assembly in human SGC7901 cells assessed as abnormal mitotic spindle formation at 2 fold IC50 level incubated for 48 hrs by immunofluorescence staining based fluorescence microscopy	25262051
human SKBR3 cells		Growth inhibition assay	48 h	Growth inhibition of human SKBR3 cells after 48 hrs by MTT assay	22850214
human SK-N-SH cells		Growth inhibition assay		Growth inhibition of human SK-N-SH cells by Sulforhodamine B assay, GI50=0.66 μM	21106458
human SKOV3 cells		Proliferation assay	48 h	Antiproliferative activity against human SKOV3 cells after 48 hrs by alamar blue assay, IC50=80 nM	21563750
human SW480 cells		Proliferation assay	48 h	Antiproliferative activity against human SW480 cells after 48 hrs by alamar blue assay, IC50=0.12 μM	21563750
human U2OS cells		Function assay	72 h	Induction of mitotic arrest in human U2OS cells assessed as mitotic index after 72 hrs by microscopic analysis, EC50=50 nM	24900887
K562 cells		Function assay		Effective concentration required to arrest propidium iodide stained K562 cells in G2/M phase, EC50=82 nM	12852768
L1210 murine leukemia cell		Growth inhibition assay		In vitro for its inhibitory activity against L1210 murine leukemia cell growth, IC50=38 nM	1738146
LT12 cells		Proliferation assay	48 h	Antiproliferative activity against LT12 cells by XTT assay after 48 hrs, IC50=40 nM	17181164
lymphoid leukemia L1210 cells		Proliferation assay	48 h	Inhibitory concentration against proliferation of cultured lymphoid leukemia L1210 cells during 48 hrs, ID50=27 nM	7131483
mouse NIH3T3 cells	0.5 ug/ml	Function assay	24 h	Induction of apoptosis in mouse NIH3T3 cells assessed as accumulation at subG1 phase at 0.5 ug/ml after 24 hrs by flow cytometry	18247573
mouse P388 cells		Proliferation assay	48 h	Antiproliferative activity against mouse P388 cells after 48 hrs by XTT assay, IC50=40 nM	19220018
rat LT12 cells		Proliferation assay	48 h	Antiproliferative activity against rat LT12 cells after 48 hrs by XTT assay, IC50=6 nM	19220018

诺考达唑 /Nocodazole {[allProObj[0].p_purity_real_show]}

Nocodazole 纯度/质量文件产品仅供科研

全球学术期刊中引用的产品

Nocodazole 质控信息

Nocodazole 生物活性

Nocodazole 细胞研究

Nocodazole 动物研究

Nocodazole 参考文献

Nocodazole 实验方案

Nocodazole 技术信息

最近浏览的产品

大规格询价

摩尔计算器

靶点

总药量计算器

工作液计算器

细胞研究

临床研究

确认信息

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

诺考达唑 /Nocodazole {[allProObj[0].p_purity_real_show]}

Nocodazole 纯度/质量文件 产品仅供科研

全球学术期刊中引用的产品

Nocodazole 质控信息

Nocodazole 生物活性

Nocodazole 细胞研究

Nocodazole 动物研究

Nocodazole 参考文献

Nocodazole 实验方案

Nocodazole 技术信息

最近浏览的产品

大规格询价

摩尔计算器

靶点

总药量计算器

工作液计算器

细胞研究

临床研究

确认信息

请登录您的专属账户

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

Nocodazole 纯度/质量文件产品仅供科研