Hydroxyurea是一种细胞凋亡诱导剂，通过靶向核苷酸还原酶抑制 DNA 合成，还显示出抗正痘病毒活性。
HazMat Fee +
There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Ribonucleotide reductase (RNR) is an emzyme that catalyses the rate limiting step in the production of deoxyribonucleotides needed for DNA synthesis[1]. RNR inhibitors are a family of anti-cancer drugs because they could affect the growth of tumor cells by interfering the DNA synthesis. Hydroxyurea (HU) is the inhibitor of ribonucleotide reductase (RNR) with IC50 value of 150 μM[2]. The ML L5178 cells, HU treatment at concentrations 0.25 mmol/l resulted in significant increases of DNA damage, detected as strand breaks in the alkaline comet assay [3]. After incubation with 2 mM HU for 4 hours on MCF7 cells, the level of phosphorylated RPA2 measured significantly increased and for 6 hours the percentage of cells with ssDNA was markedly decreased[4]. The pregnant C57BL/6 mice was intraperitoneally injected with 225 mg/kg HU and their embryos were explanted later. The RNR activity decreased significantly after 1 hour and the dNTP level in the brain tissues was attenuated with time[5].
作用机制
The HU is converted into its oxidized form compound III in the radical site of the ribonucleotide reductase protein. The compound III then reacts with the active site residues specially Tyr-176, and inhibits the radical generation and subsequently inhibits the RNR enzyme[6].
Hydroxyurea 细胞研究
细胞系
浓度
检测类型
检测时间
活动说明
数据源
L1210 Leukemia cells
Growth inhibition assay
Concentration required for 50% inhibition of cell growth (L1210 Leukemia), IC50=21.3796 μM
2991520
P388 leukemic cell
Proliferation assay
24-48 h
Antiproliferative activity of compound expressed as concentration that inhibits 50% of growth of P388 leukemic cell suspension from 24 to 48 hr after compound addition, IC50=32 μM
Concentration required for 50% inhibition of cell growth (L1210 Leukemia), IC50=21.3796 μM
2991520
P388 leukemic cell
Proliferation assay
24-48 h
Antiproliferative activity of compound expressed as concentration that inhibits 50% of growth of P388 leukemic cell suspension from 24 to 48 hr after compound addition, IC50=32 μM
搜索
HazMat Fee +
