Anisomycin is a naturally occuring antibiotic which can act as a potent activator of stress-activated protein kinases (JNK/SAPK) and p38 MAPK, and also can inhibit protein synthesis by blocking translation.
c-Jun N-terminal kinase (JNK) is a member of the mitogen-activated protein kinases (MAPK) family. Anisomycin is an activator of both JNK and p38[3]. The incubation of HL-1 myocytes with 50ng/mL anisomycin for 24 h markedly increased the protein level of CaMKIIδ by ∼60% relative to the control group. The c-jun phosphorylation in total cell lysates and nuclear extracts was also increased by the treatment of 50ng/mL HL-1 myocytes with anisomycin for 24 h[4]. The intraperitoneal injection of wild-type, TNFR p55/p75-/-, and Nox2-/- mice with 0.1mg/kg anisomycin resulted in the activation of p38, an increase in the production of TNF-α and Nox2, and the phosphorylation of Nox2 compared with the control group[5].
Anisomycin 细胞研究
细胞系
浓度
检测类型
检测时间
活动说明
数据源
HEK293 cells
Cytotoxic assay
Inhibitory concentration required to produce cytotoxicity against HEK293 cells, IC50=0.02 μM
16005213
human HeLa cells
10 μM
Function assay
Inhibition of translation in human HeLa cells at 10 uM by 35S-methionine metabolic labeling study
15165136
mouse RAW264.7 cells
5 μM
Function assay
30 mins
Activation of p38MAPK in mouse RAW264.7 cells assessed as phosphorylation at Thr180/Tyr182 at 5 uM after 30 mins by Western blotting analysis
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