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Anisomycin

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Chemical Structure| 22862-76-6 同义名 : Flagecidin;Wuningmeisu C;AI3-50846;NSC 76712
CAS号 : 22862-76-6
货号 : A646979
分子式 : C14H19NO4
纯度 : 99%+
分子量 : 265.305
MDL号 : MFCD00077650
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(188.46 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 4 mg/mL(15.08 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：

2% DMSO+corn oil 5 mg/mL

生物活性
描述 c-Jun N-terminal kinase (JNK) is a member of the mitogen-activated protein kinases (MAPK) family. Anisomycin is an activator of both JNK and p38[3]. The incubation of HL-1 myocytes with 50ng/mL anisomycin for 24 h markedly increased the protein level of CaMKIIδ by ∼60% relative to the control group. The c-jun phosphorylation in total cell lysates and nuclear extracts was also increased by the treatment of 50ng/mL HL-1 myocytes with anisomycin for 24 h[4]. The intraperitoneal injection of wild-type, TNFR p55/p75-/-, and Nox2-/- mice with 0.1mg/kg anisomycin resulted in the activation of p38, an increase in the production of TNF-α and Nox2, and the phosphorylation of Nox2 compared with the control group[5].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
HEK293 cells Cytotoxic assay Inhibitory concentration required to produce cytotoxicity against HEK293 cells, IC50=0.02 μM 16005213
human HeLa cells 10 μM Function assay Inhibition of translation in human HeLa cells at 10 uM by 35S-methionine metabolic labeling study 15165136
mouse RAW264.7 cells 5 μM Function assay 30 mins Activation of p38MAPK in mouse RAW264.7 cells assessed as phosphorylation at Thr180/Tyr182 at 5 uM after 30 mins by Western blotting analysis 23294286
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.77mL

0.75mL

0.38mL

18.85mL

3.77mL

1.88mL

37.69mL

7.54mL

3.77mL
参考文献

[1]Dubue JD, McKinney TL, et al. Intrahippocampal Anisomycin Impairs Spatial Performance on the Morris Water Maze. J Neurosci. 2015 Aug 5;35(31):11118-24.

[2]Dubue JD, McKinney TL, et al. Intrahippocampal Anisomycin Impairs Spatial Performance on the Morris Water Maze. J Neurosci. 2015 Aug 5;35(31):11118-24.

[3]Nikaido M, Otani T, Kitagawa N, et al. Anisomycin, a JNK and p38 activator, suppresses cell-cell junction formation in 2D cultures of K38 mouse keratinocyte cells and reduces claudin-7 expression, with an increase of paracellular permeability in 3D cultures. Histochem Cell Biol. 2019;151(5):369-384. doi:10.1007/s00418-018-1736-z

[4]Gao X, Wu X, Yan J, et al. Transcriptional regulation of stress kinase JNK2 in pro-arrhythmic CaMKIIδ expression in the aged atrium. Cardiovasc Res. 2018;114(5):737-746. doi:10.1093/cvr/cvy011

[5]Zhao TC, Zhang L, Liu JT, Guo TL. Disruption of Nox2 and TNFRp55/p75 eliminates cardioprotection induced by anisomycin. Am J Physiol Heart Circ Physiol. 2012;303(10):H1263-H1272. doi:10.1152/ajpheart.00306.2012