Y-27632 dihydrochloride | Y-27632 (hydrochloride);Y-27632 2HCl | Organoid Culture Additive | ROCK

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Y-27632二盐酸盐 /Y-27632 dihydrochloride 99%+

货号：A101744 同义名： Y-27632 (hydrochloride);Y-27632 2HCl

Y-27632 dihydrochloride 是一种 ATP 竞争性的 ROCK 抑制剂，具有口服活性。Y-27632 dihydrochloride (GMP) 具有抗癫痫作用，可用于细胞研究生产的辅助试剂。

Y-27632 dihydrochloride 化学结构
CAS号：129830-38-2

Y-27632 dihydrochloride 3D分子结构
CAS号：129830-38-2

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Y-27632 dihydrochloride 纯度/质量文件产品仅供科研

货号：A101744 标准纯度： 99%+ 产品说明书

批次查询：批次纯度: COA SDS NMR HPLC CNMR LC-MS

全球学术期刊中引用的产品: • Cell,2024. Ambeed. [ A125712 ]; • Cancer Discov., 2024. Ambeed. [ A285925 ]; • JMC, 2024, 67(17): 14974-14985. Ambeed. [ A288696 ]; • JMC, 2024, 67(17): 15061-15079. Ambeed. [ A524399 , A633512 , A144280 , A174613 ]; • EJNMMI Radiopharm. Chem., 2024, 9, 61. Ambeed. [ A1257961 , A622068 ]; 更多 >

ROCK 亚型比较

产品名称	ROCK ↓ ↑	ROCK1 ↓ ↑	ROCK2 ↓ ↑	其他靶点	纯度
Thiazovivin	+ ROCK, IC50: ~0.5 μM				98%
Netarsudil hydrochloride	++++ ROCK, Ki: 2 nM				99%+
Y-33075 2HCl	++++ ROCK, IC50: 3.6 nM				99%+
ROCK1-IN-1	++ ROCK, Ki: 17nM				95%
GSK429286A		+++ ROCK1, IC50: 14 nM	++ ROCK2, IC50: 63 nM		99%+
Ripasudil		++ ROCK1, IC50: 51 nM	++ ROCK2, IC50: 19 nM		99%+
Belumosudil			++ ROCK2, IC50: 60 nM ROCK2, Ki: 41 nM		99%+
Y-27632 dihydrochloride		++ ROCK1, Ki: 140 nM	+ ROCK2, Ki: 300 nM		99%+
Fasudil HCl			+ ROCK2, Ki: 330 nM	Rho,PKG	98%
Chroman 1		++++ ROCK1, IC50: 52 pM	++++ ROCK2, IC50: 1 pM		99%
BAY-549		++++ ROCK1, IC50: 0.6 nM	++++ ROCK2, IC50: 1.1 nM		99%+
AT13148		+++ ROCK1, IC50: 6 nM	+++ ROCK2, IC50: 4 nM	PKA	98+%
RKI-1447		+++ ROCK1, IC50: 14.5 nM	+++ ROCK2, IC50: 6.2 nM		99%+
Hydroxyfasudil HCl		+ ROCK1, IC50: 0.73 μM	+ ROCK2, IC50: 0.72 μM	PKA	98%
H-1152 2HCl			+++ ROCK2, IC50: 0.0120 μM	PKG	99%
GSK269962A HCl		++++ ROCK1, IC50: 1.6 nM	+++ ROCK2, IC50: 4 nM		99%+
1. 鼠标悬停在“+”上可以显示相关IC50的具体数值。"+"越多，抑制作用越强。2. "✔"表示该化合物对相应的亚型有抑制作用，但抑制强度暂时没有相关数据。

Y-27632 dihydrochloride 生物活性

靶点	ROCK1 ROCK1 (p160ROCK), Ki:140 nM ROCK2 ROCK2, Ki:300 nM ROCK1 ROCK1, Ki:140 nM
描述	Y-27632 is a specific inhibitor of Rho-associated kinases^[1]. Y-27632 2HCl is the dihydrochloride form of Y-27632 with much higher solubility than Y-27632. It is usually used for ES cell maintance^[2], cell reprogramming^[3],improving cell suviving after thawing^[4], as well as facilitates cell proliferation and migration^[5].
作用机制	Y-27632 2HCl can inhibit ROCK1 and ROCK2 in an ATP-competition manner.^[1]

Y-27632 dihydrochloride 细胞研究

细胞系	浓度	检测类型	检测时间	活动说明	数据源
C2C12	10 μM	Function Assay	6 h	Prevents the serine phosphorylation of IRS-1 induced by insulin and/or TNF-α	16267124
CCL39	30 μM	Function Assay	30 min	Completely abolishes activation of Na-H exchanger NHE1 by integrins	9693382
Cynomolgus monkey embryonic stem cells	20 µM	Cytotoxic Assay	24 h	Promotes cyES cell survival	18940855
Dbl-d	10 μM	Growth Inhibition Assay	18 d	Strongly inhibits cell growth	10021386

Y-27632 dihydrochloride 动物研究

Dose	Mice: min = 1 mg/kg^[8], max = 40 mg/kg^[9] Rats: min = 1 mg/kg^[10], max = 100 mg/kg^[9]
Administration	p.o., i.v., i.p.

Y-27632 dihydrochloride 参考文献

[1]Ishizaki T, Uehata M, et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol. 2000 May;57(5):976-83.

[2]Li X, Meng G, et al. The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation. Stem Cells Dev. 2008 Dec;17(6):1079-85.

[3]Zhao Y, Zhao T, et al. A XEN-like State Bridges Somatic Cells to Pluripotency during Chemical Reprogramming. Cell. 2015 Dec 17;163(7):1678-91.

[4]Gauthaman K, Fong CY, et al. ROCK inhibitor Y-27632 increases thaw-survival rates and preserves stemness and differentiation potential of human Wharton's jelly stem cells after cryopreservation. Stem Cell Rev. 2010 Dec;6(4):665-76.

[5]Wang T, Kang W, et al. Rho-kinase inhibitor Y-27632 facilitates the proliferation, migration and pluripotency of human periodontal ligament stem cells. J Cell Mol Med. 2017 Nov;21(11):3100-3112.

[6]Osei-Owusu P, Knutsen RH, et al. Altered reactivity of resistance vasculature contributes to hypertension in elastin insufficiency. Am J Physiol Heart Circ Physiol. 2014;306(5):H654-66.

[7]Mavria G, Vercoulen Y, et al. ERK-MAPK signaling opposes Rho-kinase to promote endothelial cell survival and sprouting during angiogenesis. Cancer Cell. 2006;9(1):33-44.

Y-27632 dihydrochloride 实验方案

计算器

存储液制备

1mg

5mg

10mg

1 mM

5 mM

10 mM

3.12mL

0.62mL

0.31mL

15.61mL

3.12mL

1.56mL

31.22mL

6.24mL

3.12mL

Y-27632 dihydrochloride 技术信息

CAS号	129830-38-2
分子式	C₁₄H₂₃Cl₂N₃O
分子量	320.258

别名	Y-27632 (hydrochloride);Y-27632 2HCl
运输	蓝冰

存储条件

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月

溶解度

DMSO: 35 mg/mL(109.29 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H₂O: 100 mg/mL(312.25 mM)，配合低频超声助溶

动物实验配方

PO 0.5% CMC-Na 40 mg/mL clear

细胞研究

细胞系	浓度	检测类型	检测时间	活动说明	数据源

C2C12	10 μM	Function Assay	6 h	Prevents the serine phosphorylation of IRS-1 induced by insulin and/or TNF-α	16267124
CCL39	30 μM	Function Assay	30 min	Completely abolishes activation of Na-H exchanger NHE1 by integrins	9693382
Cynomolgus monkey embryonic stem cells	20 µM	Cytotoxic Assay	24 h	Promotes cyES cell survival	18940855
Dbl-d	10 μM	Growth Inhibition Assay	18 d	Strongly inhibits cell growth	10021386
Dbl-e	10 μM	Growth Inhibition Assay	18 d	Moderately inhibits cell growth	10021386
HCT116	10 μM	Growth Inhibition Assay	18 d	Strongly inhibits cell growth	10021386
HCT15	10 μM	Growth Inhibition Assay	18 d	Does not inhibit cell growth	10021386
HeLa	10 μM	Function Assay	30 min	Inhibits the formation of stress fibers and the assembly of vinculin-containing focal adhesions	9668072
HT22	10 µM	Cytotoxic Assay	13 h	Protects against glutamate-induced neuronal death	22810835
LNCaP	25 μM	Growth Inhibition Assay	17 h	Does not inhibit cell growth	10720471
LS174T	10 μM	Growth Inhibition Assay	18 d	Moderately inhibits cell growth	10021386
Mesothelial cells from rat mesentery	30 μM	Invasive Assay	20 h	Blocks invasive activity	9930872
mNET1-d	10 μM	Growth Inhibition Assay	18 d	Strongly inhibits cell growth	10021386
mNET1-e	10 μM	Growth Inhibition Assay	18 d	Strongly inhibits cell growth	10021386
N1E-115	10 μM	Function Assay	2 h	Inhibits the assembly of microtubules and intermediate filaments to form extended processes	9647654
Neonatal rat ventricular myocytes	10 μM	Function Assay	48 h	Inhibits ET-1-induced increases in protein synthesis, cell size and myofibrillar organization	10386613
NIH3T3	10 μM	Growth Inhibition Assay	18 d	Does not inhibit cell growth	10021386
NIH3T3	10 μM	Growth Inhibition Assay	18 d	Does not inhibit cell growth	10021386
Pancreatic acinar cells	10 μM	Function Assay	70 min	Potentiates CCK-stimulated pancreatic enzyme secretion	12745080
PC 12	10 μM	Function Assay	24 h	Attenuates catecholamine biosynthesis	16219424
PC3	25 μM	Function Assay	1 h	Induces morphological changes	10720471
PC3	25 μM	Migration Assay	1 h	Inhibits the BMFB-CM and the EGF-stimulated migration	10720471
PC3	25 μM	Growth Inhibition Assay	17 h	Does not inhibit cell growth	10720471
Ras-2	10 μM	Growth Inhibition Assay	18 d	Strongly inhibits cell growth	10021386
Ras-4	10 μM	Growth Inhibition Assay	18 d	Strongly inhibits cell growth	10021386
Rat hepatic stellate cells	30 μM	Function Assay	48 h	Diminishes the phosphorylation of Erk2, and decreases new DNA synthesis	10845663
Rat Vascular Smooth Muscle Cells	10 μM	Function Assay	2 h	Inhibits angiotensin II-induced hypertrophy	10642317
Salivary gland stem cells	10 µM	Function Assay	7 d	Reduces SGSC senescence	25804560
Src-1	10 μM	Growth Inhibition Assay	18 d	Does not inhibit cell growth	10021386
Src-1	10 μM	Growth Inhibition Assay	18 d	Does not inhibit cell growth	10021386
Src-2	10 μM	Growth Inhibition Assay	18 d	Does not inhibit cell growth	10021386
Src-4	10 μM	Growth Inhibition Assay	18 d	Does not inhibit cell growth	10021386
Stellate Cell	25 μM	Function Assay	15 min	Inhibits formation of F-actin stress fibers and phosphorylation of myosin light chain	10600496
SW620	10 μM	Growth Inhibition Assay	18 d	Does not inhibit cell growth	10021386
Swiss 3T3 cells	10 μM	Function Assay	2 h	Inhibits the assembly of microtubules and intermediate filaments to form extended processes	9647654
Swiss3T3	10 µM	Colony-forming Assay	13 d	Increases prostate cell colony-forming activity	21464902
TSGH 8301	20 µM	Migration Assay	1 h	Increases cell migration	19896475

Y-27632二盐酸盐 /Y-27632 dihydrochloride 99%+

Y-27632 dihydrochloride 纯度/质量文件产品仅供科研

全球学术期刊中引用的产品

Y-27632 dihydrochloride 质控信息

Y-27632 dihydrochloride 生物活性

Y-27632 dihydrochloride 细胞研究

Y-27632 dihydrochloride 动物研究

Y-27632 dihydrochloride 参考文献

Y-27632 dihydrochloride 实验方案

Y-27632 dihydrochloride 技术信息

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ROCK1	ROCK1 (p160ROCK), Ki:140 nM
ROCK2	ROCK2, Ki:300 nM
ROCK1	ROCK1, Ki:140 nM

Y-27632二盐酸盐 /Y-27632 dihydrochloride 99%+

Y-27632 dihydrochloride 纯度/质量文件 产品仅供科研

全球学术期刊中引用的产品

Y-27632 dihydrochloride 质控信息

Y-27632 dihydrochloride 生物活性

Y-27632 dihydrochloride 细胞研究

Y-27632 dihydrochloride 动物研究

Y-27632 dihydrochloride 参考文献

Y-27632 dihydrochloride 实验方案

Y-27632 dihydrochloride 技术信息

最近浏览的产品

大规格询价

摩尔计算器

靶点

总药量计算器

工作液计算器

细胞研究

临床研究

确认信息

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Y-27632 dihydrochloride 纯度/质量文件产品仅供科研