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N-乙基马来酰亚胺 /N-Ethylmaleimide {[allProObj[0].p_purity_real_show]}

货号:A175883 同义名: N-乙基顺丁烯二酰亚胺 / NEM;NSC 7638 Ambeed 开学季,买赠积分,赢豪礼

N-Ethylmaleimide is an irreversible inhibitor of all cysteine peptidases, it's an organic compound derived from maleic acid.

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Type HazMat fee for 500 gram (Estimated)
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N-Ethylmaleimide 化学结构 CAS号:128-53-0
N-Ethylmaleimide 化学结构
CAS号:128-53-0
N-Ethylmaleimide 3D分子结构
CAS号:128-53-0
N-Ethylmaleimide 化学结构 CAS号:128-53-0
N-Ethylmaleimide 3D分子结构 CAS号:128-53-0
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N-Ethylmaleimide 纯度/质量文件 产品仅供科研

货号:A175883 标准纯度: {[allProObj[0].p_purity_real_show]}
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产品名称 Cysteine Protease 其他靶点 纯度
Z-FA-FMK {[allProObj[0].p_purity_real_show]}
Leupeptin hemisulfate {[allProObj[0].p_purity_real_show]}
PMSF {[allProObj[0].p_purity_real_show]}
PD 151746 +

μ-Calpain, IC50: 260 nM

m-calpain, IC50: 5.33 μM

{[allProObj[0].p_purity_real_show]}
Odanacatib ++++

Cathepsin K (human), IC50: 0.2 nM

Cathepsin K (rabbit), IC50: 1 nM

{[allProObj[0].p_purity_real_show]}
E-64 +++

Cysteine protease, IC50: 9 nM

{[allProObj[0].p_purity_real_show]}
E 64c {[allProObj[0].p_purity_real_show]}
E-64d {[allProObj[0].p_purity_real_show]}
MG-101 {[allProObj[0].p_purity_real_show]}
Calpeptin ++

Calpain I (porcine erythrocytes), ID50: 52 nM

Calpain II (porcine kidney), ID50: 40 nM

{[allProObj[0].p_purity_real_show]}
Cathepsin inhibitor 1 +++

Cathepsin L2, pIC50: 5.5

Cathepsin L, pIC50: 7.9

{[allProObj[0].p_purity_real_show]}
1. 鼠标悬停在“+”上可以显示相关IC50的具体数值。"+"越多,抑制作用越强。2. "✔"表示该化合物对相应的亚型有抑制作用,但抑制强度暂时没有相关数据。

N-Ethylmaleimide 生物活性

描述 N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor[2]. N-ethylmaleimide (NEM) was shown to be a highly effective inhibitor of the following coupled mitochondrial processes: oxidative phosphorylation, ATP-32Pi exchange, Pi-induced light scattering and configurational changes, State III respiration, valinomycin-induced translocation of potassium with Pi as the anion, and calcium accumulation in presence of Pi. Also NEM, even et levels 2-3 times greater than those required to inhibit oxidative phosphorylation in intact mitochondria, did not inhibit coupled processes in submitochondrial particles[3]. When D. salina thylakoid membranes are treated with N-ethylmaleimide, both photophosphorylation and the inducible CF1 ATPase activity are partially (approx. 60%) inhibited. Treatment of the purified, latent, D. salina CF1 with low concentrations of N-ethylmaleimide also results in the partial (approx. 60%) inhibition of the inducible ATPase activity (IC50 approximately 50 mM). N-ethylmaleimide-induced inhibition of the ATPase activity of either membrane-bound or solubilized CF1 is partially reversed by either prolonged incubation at low concentrations of N-ethylmaleimide or short incubation times at high concentrations of N-ethylmaleimide[4].

N-Ethylmaleimide 参考文献

[2]Wu KH, Tai PC. Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolytic activity of CvaB, an ATP-binding cassette transporter. J Biol Chem. 2004 Jan 9;279(2):901-9

[3]Specific inhibition of phosphate transport in mitochondria by N-ethylmaleimide

[4]Selman-Reimer S, Duhe RJ, Selman BR. N-Ethylmaleimide inhibition of the catalytic activities of the Dunaliella salina coupling factor 1 (CF1) and the restoration of the inhibition of the CF1 ATPase activity by N-ethylmaleimide. Biochim Biophys Acta. 1985 Dec 16;810(3):325-31

N-Ethylmaleimide 实验方案

计算器
存储液制备 1mg 5mg 10mg

1 mM

5 mM

10 mM

7.99mL

1.60mL

0.80mL

39.96mL

7.99mL

4.00mL

79.92mL

15.98mL

7.99mL

N-Ethylmaleimide 技术信息

CAS号128-53-0
分子式C6H7NO2
分子量 125.13
别名 N-乙基顺丁烯二酰亚胺 ;NEM;NSC 7638
运输蓝冰
存储条件

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月

溶解度

DMSO: 50 mg/mL(399.58 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

H2O: 50 mg/mL(399.58 mM),配合低频超声助溶

无水乙醇: 12 mg/mL(95.9 mM),配合低频超声助溶,注意:无水乙醇开封后,易挥发,也会吸收空气中的水分,导致溶解能力下降,请避免使用开封较久的乙醇

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