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N-Ethylmaleimide

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Chemical Structure| 128-53-0 同义名 : N-乙基顺丁烯二酰亚胺 ;NEM;NSC 7638
CAS号 : 128-53-0
货号 : A175883
分子式 : C6H7NO2
纯度 : 97%
分子量 : 125.13
MDL号 : MFCD00005509
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(399.58 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 50 mg/mL(399.58 mM)，配合低频超声助溶

无水乙醇: 12 mg/mL(95.9 mM)，配合低频超声助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇
动物实验配方：
生物活性
描述 N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor[2]. N-ethylmaleimide (NEM) was shown to be a highly effective inhibitor of the following coupled mitochondrial processes: oxidative phosphorylation, ATP-32Pi exchange, Pi-induced light scattering and configurational changes, State III respiration, valinomycin-induced translocation of potassium with Pi as the anion, and calcium accumulation in presence of Pi. Also NEM, even et levels 2-3 times greater than those required to inhibit oxidative phosphorylation in intact mitochondria, did not inhibit coupled processes in submitochondrial particles[3]. When D. salina thylakoid membranes are treated with N-ethylmaleimide, both photophosphorylation and the inducible CF1 ATPase activity are partially (approx. 60%) inhibited. Treatment of the purified, latent, D. salina CF1 with low concentrations of N-ethylmaleimide also results in the partial (approx. 60%) inhibition of the inducible ATPase activity (IC50 approximately 50 mM). N-ethylmaleimide-induced inhibition of the ATPase activity of either membrane-bound or solubilized CF1 is partially reversed by either prolonged incubation at low concentrations of N-ethylmaleimide or short incubation times at high concentrations of N-ethylmaleimide[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

7.99mL

1.60mL

0.80mL

39.96mL

7.99mL

4.00mL

79.92mL

15.98mL

7.99mL
参考文献

[2]Wu KH, Tai PC. Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolytic activity of CvaB, an ATP-binding cassette transporter. J Biol Chem. 2004 Jan 9;279(2):901-9

[3]Specific inhibition of phosphate transport in mitochondria by N-ethylmaleimide

[4]Selman-Reimer S, Duhe RJ, Selman BR. N-Ethylmaleimide inhibition of the catalytic activities of the Dunaliella salina coupling factor 1 (CF1) and the restoration of the inhibition of the CF1 ATPase activity by N-ethylmaleimide. Biochim Biophys Acta. 1985 Dec 16;810(3):325-31