Forskolin | Coleonol;Colforsin;NSC 375489;NSC 357088;L 75-1362B;HL 362 | 佛司可林 | Organoid Culture Additive | cAMP

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毛喉素 /Forskolin {[allProObj[0].p_purity_real_show]}

货号：A226193 同义名： 佛司可林 / Coleonol;Colforsin

Forskolin is a ubiquitous activator of eukaryotic adenylyl cyclase (AC) in a wide variety of cell types with binding (IC50=41 nM) to and activation (EC50=0.5 μM) of type I adenylyl cyclase. It is commonly used to raise levels of cAMP in the research of cell physiology.

Forskolin 化学结构
CAS号：66575-29-9

Forskolin 3D分子结构
CAS号：66575-29-9

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Forskolin 纯度/质量文件产品仅供科研

货号：A226193 标准纯度： {[allProObj[0].p_purity_real_show]} 产品说明书

批次查询：批次纯度: COA SDS NMR HPLC CNMR LC-MS

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cAMP 亚型比较

产品名称	AC ↓ ↑	EPAC1 ↓ ↑	EPAC2 ↓ ↑	纯度
Bithionol	+ sAC, IC50: 4.0 μM			98%
SQ22536	✔			99%+
ESI-09		++ EPAC1, IC50: 3.2 μM	+++ EPAC2, IC50: 1.4 μM	98%
HJC0350			+++ EPAC2, IC50: 0.3 μM	97%
1. 鼠标悬停在“+”上可以显示相关IC50的具体数值。"+"越多，抑制作用越强。2. "✔"表示该化合物对相应的亚型有抑制作用，但抑制强度暂时没有相关数据。

Forskolin 生物活性

描述

Forskolin is a strong adenylate cyclase activator with an IC50 of 41 nM. Forskolin can activate adenylyl cyclase in broken cell preparations as well as in intact tissues, thereby resulting in marked increases of intracellular cyclic adenosine phosphate (cAMP) accumulation^[1]. cAMP as a second messenger plays very important roles in biological processes. Forskolin elicits cellular responses which have been proposed to be dependent on cyclic AMP. In vitro studies have shown that forskolin significantly stimulated vasopressin (AVP) and oxytocin (OT) secretion from the rat neurohypophysis in a dose-dependent manner. Moreover, addition of forskolin to the rat hypotha-lamic SON in vitro increase cAMP accumulation, and Forskolin elicited a 3-fold greater increase in cyclic AMP in the neural lobe of the pituitary in vitro^[2].
In human mesenchymal stromal cells (hMSCs), the use of Forskolin in different concentrations inhibits cell proliferation. At the same time, Forskolin induced alkaline phosphatase levels, bone-specific target genes, and secretion of insulin-like growth factor-1 in a manner related to concentration. The effect of forskolin on in vivo shows that bone formation ability significantly enhance after treatment of hMSCs with forskolin. In addition, 0.10mM forskolin displayed larger amounts of bone by pretreating hMSCs before implantation, compared with with 0.15 mM forskolin^[3]. Importantly, Forskolin has been shown to have a number of relevant anticancer effects, such as the induction of mesenchymal-to-epithelial transition, the inhibition of proliferation, motility, and migration in many types of cancer cells, indicating its perspectives as an effective anticancer agent^[4].

作用机制

Forskolin is a strong adenylate cyclase activator via an interaction with the catalytic subunit of adenylate cyclase, directly activating the adenylate cyclase enzyme and raising intracellular cAMP levels.

Forskolin 细胞研究

细胞系	浓度	检测类型	检测时间	活动说明	数据源
3T3-L1	2.5/5 μM	Function Assay	24 h	significantly decreases ATGL protein expression at all doses tested	25590597
3T3-L1 preadipocytes	10 μM	Function Assay	12 h	induces CREB phosphorylation and C/EBPβ expression	25928058
AML-12	20 μM	Function Assay	3 h	upregulates the phosphorylation levels at Thr-411 and Ser-493	26048985
AML-12	20 μM	Function Assay	1-8 h	increases glucose production	26048985

Forskolin 动物研究

Dose	Rat: 10 mg/kg - 30 mg/kg^[5] (intragastric) Mice: 2.5 mg/kg - 10 mg/kg^[6] (p.o.), 100 mg/kg^[7] (p.o.)
Administration	Intragastric, p.o.

Forskolin 临床研究

NCT号	适应症或疾病	临床期	招募状态	预计完成时间	地点
NCT03390985	-		Completed	-	-
NCT02586883	Idiopathic Dilation of the Bro... 展开 >>nchi 收起 <<	Not Applicable	Recruiting	April 2020	France ... 展开 >> Necker-Enfants Malades Hospital Recruiting Paris, France, 75015 Contact: Isabelle SERMET-GAUDELUS, MD, PhD +33144494887 isabelle.sermet@nck.aphp.fr 收起 <<
NCT03455153	-		Completed	-	United Kingdom ... 展开 >> Lindum Medical Practice Lincoln, Lincolnshire, United Kingdom, LN2 2JP Nettleham Medical Practice Lincoln, Lincolnshire, United Kingdom, LN2 2RS Birchwood Medical Practice Lincoln, Lincolnshire, United Kingdom, LN6 0QQ 收起 <<

Forskolin 参考文献

[1]Seamon KB, Padgett W, Daly JW. Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells. Proc Natl Acad Sci U S A. 1981;78(6):3363-7.

[2]Meeker RB, Michels KM, Hayward JN. Vasopressin and oxytocin regulation of cyclic AMP accumulation in rat hypothalamo-neurohypophysial explants in vitro. Neurosci Lett. 1990;114(2):225-30.

[3]Doorn J, Siddappa R, et al. Forskolin enhances in vivo bone formation by human mesenchymal stromal cells. Tissue Eng Part A. 2012;18(5-6):558-67.

[4]Sapio L, Gallo M, et al. The Natural cAMP Elevating Compound Forskolin in Cancer Therapy: Is It Time?. J Cell Physiol. 2017;232(5):922-927.

Forskolin 实验方案

计算器

存储液制备

1mg

5mg

10mg

1 mM

5 mM

10 mM

2.44mL

0.49mL

0.24mL

12.18mL

2.44mL

1.22mL

24.36mL

4.87mL

2.44mL

Forskolin 技术信息

CAS号	66575-29-9
分子式	C₂₂H₃₄O₇
分子量	410.5

别名	佛司可林 ;Coleonol;Colforsin;NSC 375489;NSC 357088;L 75-1362B;HL 362
运输	蓝冰

存储条件

液体 -20°C:3-6个月-80°C:12个月

粉末 Sealed in dry,Store in freezer, under -20°C

溶解度

DMSO: 105 mg/mL(255.78 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

动物实验配方

IP 2% DMSO+2% Tween80+40% PEG300+water 12 mg/mL clear

PO 0.5% CMC-Na 100 mg/mL suspension

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摩尔计算器

计算溶液所需的质量，体积或浓度。 Ambeed 摩尔浓度计算器基于以下方程式：

浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)=质量 (g)

浓度

体积

分子量

质量

*在制备母液时，请始终参考产品标签或 MSDS / COA（在产品页面可下载）上所显示的对应批次产品的分子量。

靶点

靶点	数值

总药量计算器

动物实验：给药剂量 mg/kg 动物体重 g 动物数量只每天给药次给药天

需要的总药量： mg

工作液计算器

动物实验：给药剂量 mg/kg 动物体重 g 给药体积 μL

您需要配制的工作液浓度为：mg/mL

细胞研究

细胞系	浓度	检测类型	检测时间	活动说明	数据源

3T3-L1	2.5/5 μM	Function Assay	24 h	significantly decreases ATGL protein expression at all doses tested	25590597
3T3-L1 preadipocytes	10 μM	Function Assay	12 h	induces CREB phosphorylation and C/EBPβ expression	25928058
AML-12	20 μM	Function Assay	3 h	upregulates the phosphorylation levels at Thr-411 and Ser-493	26048985
AML-12	20 μM	Function Assay	1-8 h	increases glucose production	26048985
AML-12	20 μM	Function Assay	3 h	up-regulates Pgc1a, Pepck, and G6pc mRNA levels	26048985
AML-12	20 μM	Function Assay	3 h	induces the dephosphorylation of CRTC2	26048985
BAECs	25 μM	Function Assay	24 h	enhances the activation of PPARα by 5 μM resveratrol, T4HS, or 4-PAP	25798826
BeWo	25 μM	Function Assay	24/48/72 h	leads to an increase in the expression of other fusion markers	26053549
BeWo	10 μM	Function Assay	72 h	mediates BeWo cell differentiation	25713425
BeWo	20 µM	Function Assay	48 h	increases the differentiation of BeWo cells	25566740
BeWo	20 µM	Function Assay	48 h	increases the adhesion of THP-1 monocytes	25566740
BeWo	20 µM	Function Assay	48 h	increases the beta-hCG release	25362260
BeWo	20 µM	Function Assay	48 h	downregulates the level of TMEMF16	25362260
BeWo	20 µM	Function Assay	48 h	downregulates the level of GCM-1	25362260
BeWo	20 µM	Function Assay	48 h	induces cell fusion	25184477
bovine oocytes	100 μM	Function Assay	12 h	inhibits the effect of NPPA and/or NPPC to stimulate resumption of meiosis	26051611
C6	10 μM	Function Assay	20 min	increases cAMP accumulation	25069417
Caco-2	0.1/1/10 μM	Function Assay	20 min	induces a dose-dependent increase in intracellular cAMP levels	26049102
Caco-2	0.1/1/10 μM	Function Assay	24 h	increases MRP2 protein level	26049102
EM1	15 μM	Function Assay	48 h	reduces the expression of LIF or PTGS2 in CALR- or EPAC2-silenced EM1 cells	25378661
EndoC-βH1	5 μM	Function Assay	1 h	potentiates glucose-induced insulin secretion in the presence of glucose	26028562
EndoC-βH1	5 μM	Function Assay	1 h	leads to a strong cAMP increase	26028562
GH3	1 μM	Function Assay	6-h	attenuates the correlation between PRL and Bmal1 expression	25727018
GH3	1 μM	Function Assay	6-h	induces PRL and Bmal1, but not Clock, mRNA expression	25727018
GLUTag	10 µM	Function Assay	0/2/4 h	stimulates GLP-1 secretion cotreated with IBMX	25832631
GLUTag	10 µM	Function Assay	4 h	increases the pCREB levels with the IBMX	25832631
granulosa cells	10 μM	Function Assay	12/24 h	increases the levels of RGS2 mRNA	25339105
granulosa cells	10 μM	Function Assay	12/24 h	increases the levels of reporter activity for the longest fragment (−854/+18RGS2.LUC)	25339105
granulosa cells	10 μM	Function Assay	24 h	increases the intensity of DNA/protein complex	25339105
granulosa cells	10 μM	Function Assay	24 h	increases the levels of RGS2 promoter activity	25339105
H295R	10 μM	Function Assay	48 h	increases steroid metabolites in the androgen, mineralo- and glucocorticoid pathways	25869556
H929	0-100 μM	Cell Viability Assay	72 h	induces cell death dose dependently	26306624
hADSCs	5 µM	Function Assay	30 min	increases cAMP levels	25591908
HEK293	10 μM	Function Assay	6 h	increases phosphorylation of overexpressed KLHL3 at S433	26435498
HEK293	5 µM	Function Assay	30 min	increases cAMP levels	25591908
HEK-293	35 μM	Function Assay		induces a conspicuous “inactivation” of the Kv2.1 current	25962132
HEK‐CFTR	2–50 μM	Function Assay	0-12 min	induces a dose‐dependent iodide efflux	25263207
HT-29	40 μM	Function Assay	48 h	activates PP2A	24997451
HT-29	40 μM	Growth Inhibition Assay	0-72 h	inhibits cell growth time dependently	24997451
HT-29	40 μM	Function Assay	7 d	reduces colonosphere formation capability	24997451
HT-29	40 μM	Apoptosis Assay	48 h	induces an activation of caspase 3/7	24997451
HT-29	40 μM	Apoptosis Assay	48 h	induces changes in the phosphorylation status of PP2A targets	24997451
Huh-7	0-20 μM	Function Assay	2 h	results in a dose-dependent increase in c-Myc expression at the protein and mRNA levels	25109834
INA-6	0-100 μM	Cell Viability Assay	72 h	induces cell death dose dependently	26306624
L6	40 µM	Function Assay	24 h	inhibits DMH1-induced Akt activation	25247550
LNCaP	10 μM	Function Assay	12 h	induces a dramatic increase of CREB1 activity	25548099
MDCK	10 µM	Function Assay	24 h	upregulates the expression of TGF-β1 and CTGF	26202352
MDCK	10 µM	Function Assay	24 h	inhibits the increased expression of FN caused by TGF-β1	26202352
MIN6	10 μM	Function Assay	3 h	increases D3 mRNA expression	25241124
Mo-DCs	50 μM	Function Assay	24 h	promotes IL-23 production in the supernatant of zymosan stimulated Mo-DCs	26412948
OCI-Ly1	40 μM	Function Assay	1 h	induces the increment of cAMP concentrations	25576220
OCI-Ly18	40 μM	Function Assay	1 h	induces the increment of cAMP concentrations	25576220
oocytes	5 μM	Function Assay	24 h	attenuates rh-insulin action on oocyte GVBD significantly	25707854
OPM-2	0-100 μM	Cell Viability Assay	72 h	induces cell death dose dependently	26306624
PBMC	50 μM	Function Assay	24 h	inhibits the increased secretion of TNF induced by the DPE	25866079
PC12	25 μM	Function Assay	48 h	activates cAMP	25769305
PC-3	40 µM	Function Assay	2 h	leads to PP2A activation	26023836
PC-3	40 µM	Cell Viability Assay	24/48/72 h	decreases cell viability time dependently	26023836
PCCL3	10 µM	Function Assay	24 h	enhances DuOx2 promoter transcription activity	25960956
Primary bovine chondrocytes	5μM	Growth Inhibition Assay	48 h	reverses the inhibitory effect of celecoxib on proliferation in growth plate chondrocytes	25406016
RBMECs	5 μM	Function Assay	1 h	blocks the Rac1 inactivation induced by EMAP-II	26358039
RBMECs	5 μM	Function Assay	1 h	inhibits EMAP-II-induced inactivation of Rap1	26044663
RBMECs	0.05/0.5/5 μM	Function Assay	0.25 h	increases cAMP concentration	25416651
RBMECs	5 μM	Function Assay	1 h	blocks the activation of RhoA/ROCK induced by EMAP-II	25416651
RBMECs	5 μM	Function Assay	1 h	prevents the EMAP-II-induced TEER value decrease	25416651
RBMECs	5 μM	Function Assay	1 h	prevents the increase in HRP flux across the BTB induced by EMAP-II	25416651
RBMECs	5 μM	Function Assay	1 h	inhibits the decreased of amount of ZO-1 in MFs induced by EMAP-II	25416651
RBMECs	5 μM	Function Assay	1 h	reverses the changes in ZO-1 distribution seen with EMAP-II treatment	25416651
RBMECs	5 μM	Function Assay	1 h	blocks the EMAP-II-induced change in MLC phosphorylation	25416651
RBMECs	5 μM	Function Assay	1 h	blocks the actin cytoskeleton rearrangement seen with EMAP-II treatment	25416651
RPMI 8226	0-100 μM	Cell Viability Assay	72 h	induces cell death dose dependently	26306624
SC	0.5 μM	Function Assay	24 h	mimicks the effect of cAMP analogs on O1 and MBP expression	25705874
SC	0.5 μM	Function Assay	72 h	increases both Krox-20 and O1 expression in axon-related SCs but only Krox-20	25705874
SCG	20 μM	Function Assay		reversibly suppresses IKV with a IC50 of 24.4 μM	25962132
SCG	100 μM	Function Assay		reduces the excitability of SCG neurons	25962132
SH-SY5Y	30 μM	Function Assay	30 min	significantly increases the activation of PKA	26025137
SH-SY5Y	10 μM	Function Assay	1 h	increases LUC activity	25597433
SH-SY5Y	10 μM	Function Assay	1 h	increases AGC1 mRNA level	25597433
SK-N-AS	10 μM	Cell Viability Assay	24/48 h	enhances time-dependently cellular viability	25266063
SK-N-AS	10 μM	Function Assay	24 h	increases the cAMP levels	25266063
SK-N-AS	10 μM	Function Assay	24 h	increases the expression of cyclin D1	25266063
SK-N-AS	10 μM	Function Assay	30 min	induces phosphorylation of β-catenin (ser675), p-GSK3β (ser9) and concomitant higher levels of active, unphosphorylated, β-catenin	25266063
SK-N-AS	10 μM	Function Assay	10/30/60 min	increases levels of p-β-catenin (ser675) and induces accumulation of p-β-catenin (ser675) in (peri)nuclear regions	25266063
SK-N-SH	10 μM	Cell Viability Assay	48 h	enhances SK-N-SH neuroblastoma cell viability	25266063
Spinal cords	1 μM	Function Assay	30 min	stimulates cAMP levels	26126926
SW480	40 μM	Function Assay	48 h	activates PP2A	24997451
SW480	40 μM	Growth Inhibition Assay	0-72 h	inhibits cell growth time dependently	24997451
SW480	40 μM	Function Assay	7 d	reduces colonosphere formation capability	24997451
SW480	40 μM	Apoptosis Assay	48 h	induces an activation of caspase 3/7	24997451
SW480	40 μM	Apoptosis Assay	48 h	induces changes in the phosphorylation status of PP2A targets	24997451
ThGCs	10 μM	Function Assay	4 h	augments HIF1A levels that were stimulated by CoCl2	25433027
ThGCs	10 μM	Function Assay	4 h	increases CoCl2-induced EDN2 gene expression	25433027
ThGCs	10 μM	Function Assay	3 h	inhibits the effect of H2O2 on EDN2 mRNA	25433027
THP-1	1/10 μM	Function Assay	2 h	suppresses MCP-1 production	25154882
U266	0-100 μM	Cell Viability Assay	72 h	induces cell death dose dependently	26306624
UACC-647		Function Assay		leads to a rise in cAMP levels (EC50 = 20.39 μM)	25703025
UACC-647	10 μM	Function Assay	15 min	inhibits ERK phosphorylation	25703025
UACC-647	10 μM	Function Assay	15 min	increases eEF2 phosphorylation levels	25703025
ventricular cardiomyocytes	0.01-10 μM	Function Assay		increases cAMP accumulation	25203113
ventricular cardiomyocytes	0.01-10 μM	Function Assay		evokes an inotropic response 120±15% above basal with an EC50 of 2.2 µM	25203113

临床研究

NCT号	适应症或疾病	临床期	招募状态	预计完成时间	地点

NCT03390985	-		Completed	-	-
NCT02586883	Idiopathic Dilation of the Bro... 展开 >>nchi 收起 <<	Not Applicable	Recruiting	April 2020	France ... 展开 >> Necker-Enfants Malades Hospital Recruiting Paris, France, 75015 Contact: Isabelle SERMET-GAUDELUS, MD, PhD +33144494887 isabelle.sermet@nck.aphp.fr 收起 <<
NCT03455153	-		Completed	-	United Kingdom ... 展开 >> Lindum Medical Practice Lincoln, Lincolnshire, United Kingdom, LN2 2JP Nettleham Medical Practice Lincoln, Lincolnshire, United Kingdom, LN2 2RS Birchwood Medical Practice Lincoln, Lincolnshire, United Kingdom, LN6 0QQ 收起 <<
NCT01920841	Weight Loss	Not Applicable	Withdrawn(Study Design changed... 展开 >> and will be resubmitted as a new study) 收起 <<	-	United States, Louisiana ... 展开 >> Pennington Biomedical Research Center Baton Rouge, Louisiana, United States, 70808 收起 <<
NCT03652090	-		Completed	-	-
NCT02143349	Metabolic Syndrome ... 展开 >> Obesity 收起 <<	Phase 3	Unknown	May 2015	Australia, Victoria ... 展开 >> College of Health and Biomedicine Victoria University Recruiting St. Albans, Victoria, Australia, 14428 Contact: Dr. Xiao Su +61399192318 xiao.su@vu.edu.au Contact: A/Prof Michael Mathai +61399192211 michael.mathai@vu.edu.au Principal Investigator: Dr. Xiao Su Sub-Investigator: A/Prof Michael Mathai 收起 <<
NCT00864578	-		Withdrawn(loss of interest bef... 展开 >>ore enrolment started) 收起 <<	June 2009	Italy ... 展开 >> Ophthalmology Department of the University Clinic Bari, Italy, I-70124 收起 <<
NCT02807415	-		Recruiting	June 2019	Germany ... 展开 >> Justus-Liebig-University Recruiting Gießen, Germany, 35385 Contact: Lutz Nährlich, MD 004964198557620 lutz.naehrlich@paediat.med.uni-giessen.de Contact: Claudia Rückes-Nilges, Dipl-Biol 004964198556945 claudia.rueckes-nilges@paediat.med.uni-giessen.de Principal Investigator: Lutz Nährlich, MD Sub-Investigator: Stefan Kuhnert, MD Hannover Medical School Recruiting Hannover, Germany, 30625 Contact: Burkhard Tümmler, MD PhD 00495115322920 tuemmler.burkhard@mh-hannover.de Contact: Christian Dopfer, MD 00495115326111 dopfer.christian@mh-hannover.de Principal Investigator: Burkhard Tümmler, MD PhD Sub-Investigator: Christian Dopfer, MD University of Heidelberg Recruiting Heidelberg, Germany, 69120 Contact: Marcus Mall, MD 00496221564502 Marcus.Mall@med.uni-heidelberg.de Contact: Simon Gräber, MD 004962215638926 Simon.Graeber@med.uni-heidelberg.de Principal Investigator: Marcus Mall, MD Sub-Investigator: Simon Gräber, MD Sub-Investigator: Susanne Dittrich, MD 收起 <<
NCT00863811	-		Withdrawn(loss of interest bef... 展开 >>ore enrolment started) 收起 <<	July 2009	Italy ... 展开 >> Ophthalmology Department of the University Bari, Italy, I-70124 收起 <<
NCT01254006	Glaucoma	Not Applicable	Completed	-	Italy ... 展开 >> Univerisity La Sapienza Latina, Italy 收起 <<

毛喉素 /Forskolin {[allProObj[0].p_purity_real_show]}

Forskolin 纯度/质量文件产品仅供科研

全球学术期刊中引用的产品

Forskolin 质控信息

Forskolin 生物活性

Forskolin 细胞研究

Forskolin 动物研究

Forskolin 临床研究

Forskolin 参考文献

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Forskolin 技术信息

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毛喉素 /Forskolin {[allProObj[0].p_purity_real_show]}

Forskolin 纯度/质量文件 产品仅供科研

全球学术期刊中引用的产品

Forskolin 质控信息

Forskolin 生物活性

Forskolin 细胞研究

Forskolin 动物研究

Forskolin 临床研究

Forskolin 参考文献

Forskolin 实验方案

Forskolin 技术信息

最近浏览的产品

大规格询价

摩尔计算器

靶点

总药量计算器

工作液计算器

细胞研究

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T

X

Y

Z

Forskolin 纯度/质量文件产品仅供科研