CO-releasing molecules (CO-RMs) are compounds that contains a heavy metal or iron surrounded by carbonyl (CO) group as coordinated ligands. They can produce the CO in the biological systems, which can be used to treat diseased tissues such as inflammatory diseases[1]. CORM-3 (tricarbonylchloro(glycinato)ruthenium(II)) is a synthesized water-soluble form of metal carbonyl that can release CO in physiological conditions. Raw 264.7 cells were treated with 10 μg/ml LPS and then incubated with 50 – 600 μM of CORM-3 for 24h. The amount of NO measured by the accumulation of nitrite was increased by LPS and attenuated by CORM-3 in a dose dependent manner. 48 hours after incubation of CORM-3, the production of IL-1beta measured by ELISA was diminished up to 61% at a concentration of 400 μM of CORM-3[2]. Rat H9c2 cardiac cells were incubated with 10, 25 or 50 μmol/L of CORM-3 under hypoxic conditions for 24 hours, the cell viability was recovered to 100% at 25 μmol/L of CORM-3 and above[3]. Male BALB/c mice were intraperitoneal administration of 40 mg/kg CORM-3 at 1 day and 15 minutes before cardiac harvest, and the hearts were transplanted into male CBA mice. One dose of CORM-3 was given to the recipients 1 day before surgery, 30 minutes before cardiac reperfusion, and 1 hour after transplantation. It was found that the survival rate of the mice treatement with CORM-3 was significantly higher than the control group[3].
作用机制
The biologically compatible ligand (glycine) was coordinated on the metal center of CORM-3, to promote the dissociation of the CO in biological environment[4].
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