CORM-3

产品说明书

Print
Chemical Structure| 475473-26-8 同义名 : -
CAS号 : 475473-26-8
货号 : A812133
分子式 : C5H4ClNO5Ru
纯度 : 97%
分子量 : 294.612
MDL号 : MFCD07364048
存储条件：

Pure form Inert atmosphere,Store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 80 mg/mL(271.54 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 50 mg/mL(169.71 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 CO-releasing molecules (CO-RMs) are compounds that contains a heavy metal or iron surrounded by carbonyl (CO) group as coordinated ligands. They can produce the CO in the biological systems, which can be used to treat diseased tissues such as inflammatory diseases[1]. CORM-3 (tricarbonylchloro(glycinato)ruthenium(II)) is a synthesized water-soluble form of metal carbonyl that can release CO in physiological conditions.
Raw 264.7 cells were treated with 10 μg/ml LPS and then incubated with 50 – 600 μM of CORM-3 for 24h. The amount of NO measured by the accumulation of nitrite was increased by LPS and attenuated by CORM-3 in a dose dependent manner. 48 hours after incubation of CORM-3, the production of IL-1beta measured by ELISA was diminished up to 61% at a concentration of 400 μM of CORM-3[2]. Rat H9c2 cardiac cells were incubated with 10, 25 or 50 μmol/L of CORM-3 under hypoxic conditions for 24 hours, the cell viability was recovered to 100% at 25 μmol/L of CORM-3 and above[3].
Male BALB/c mice were intraperitoneal administration of 40 mg/kg CORM-3 at 1 day and 15 minutes before cardiac harvest, and the hearts were transplanted into male CBA mice. One dose of CORM-3 was given to the recipients 1 day before surgery, 30 minutes before cardiac reperfusion, and 1 hour after transplantation. It was found that the survival rate of the mice treatement with CORM-3 was significantly higher than the control group[3].
作用机制 The biologically compatible ligand (glycine) was coordinated on the metal center of CORM-3, to promote the dissociation of the CO in biological environment[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.37mL

0.67mL

0.34mL

16.86mL

3.37mL

1.69mL

33.71mL

6.74mL

3.37mL
参考文献

[1]Abeyrathna N, Washington K, et al. Nonmetallic carbon monoxide releasing molecules (CORMs). Org Biomol Chem. 2017;15(41):8692-8699.

[2]Choi EY, Choe SH, et al. Carbon monoxide-releasing molecule-3 suppresses Prevotella intermedia lipopolysaccharide-induced production of nitric oxide and interleukin-1β in murine macrophages. Eur J Pharmacol. 2015.

[3]Clark JE, Naughton P, et al. Cardioprotective actions by a water-soluble carbon monoxide-releasing molecule. Circ Res. 2003;93(2):e2-8.

[4]Motterlini R, Clark JE, et al. Carbon monoxide-releasing molecules: characterization of biochemical and vascular activities. Circ Res. 2002;90(2):E17-24.