Treosulfan is an alkylating agent used for conventional, as well as high-dose chemotherapy regimens, whereby plasma concentrations over 500 μg/mL can be achieved.

Altretamine is an alkylating agent proposed as an antineoplastic.

Melflufen hydrochloride是Melphalan的二肽前药，作为一种烷基化剂，显示出对多发性骨髓瘤的抗肿瘤活性，能够不可逆地损伤骨髓瘤细胞的DNA并诱导细胞毒性。

CB 1954 is an anticancer prodrug used in gene therapy research and is activated by NAD(P)H Quinone Oxidoreductase 2.

Lobaplatin is a diastereometric mixture of platinum(II) complexes containing a 1,2-bis(aminomethyl)cyclobutane stable ligand and lactic acid as the leaving group. Its antitumour activity results from the formation of DNA-drug adducts, mainly as GG and AG intra-strand cross-links.

Nedaplatin is a derivative of cisplatin and DNA damage agent.

Bendamustine is a DNA cross-linking agent.

Carmustine is a cell-cycle phase nonspecific alkylating antineoplastic agent.

Carboplatin (NSC 241240)通过与DNA结合，阻碍DNA合成，从而阻碍复制和转录过程，最终导致细胞死亡。Carboplatin是从顺铂（CDDP）衍生出来的，是一种有效的抗癌剂。

Thio-TEPA is an alkylating agent used to treat cancer, this molecule features tetrahedral phosphorus and is structurally akin to phosphate.

Temozolomide is a DNA-methylating agent and an alkylating cytostatic drug used in the treatment of some brain cancers, astrocytoma and glioblastoma multiforme.

Oxaliplatin inhibits DNA synthesis by conforming DNA adducts.

Ifosfamide is a nitrogen mustard alkylating agent used in the treatment of cancer.

Procarbazine HCl, an antineoplastic chemotherapy drug, is an alkylating agent for the treatment of Hodgkin's lymphoma and certain brain cancers and also inhibits MAO thus increasing the effects of sympathomimetics, TCAs, and tyramine.

Lomustine is an alkylating nitrosourea compound used in chemotherapy. Lomustine (420 μM) triggers apoptosis through the mitochondrial pathway via decrease in the level of the anti-apoptosis proteins Bcl-2 and Bcl-xl, respectively, in both medulloblastoma and normal human epithelial and fibroblast cells. Lomustine induces cell cycle delay in G2/M phase in medulloblastoma cells and up-regulates p21 protein level in a p53-independent manner in HFSN1 cells.

RITA induced both DNA-protein and DNA-DNA cross-links with no detectable DNA single-strand breaks. It is MDM2-p53 interaction inhibitor and activates p53 expression.

Calicheamicin is a potent DNA crosslinker cytotoxic antibiotic.

Lurbinectedin是一种DNA小沟共价结合剂，具有强效抗肿瘤活性。它通过与DNA的小沟结合，干扰DNA的转录和复制过程，对多种实体瘤表现出良好的治疗效果。

Busulfan is a bifunctional alkylating agent.

Cisplatin (CDDP) 是一种抗肿瘤化疗剂，通过与 DNA 交联引起癌细胞中的 DNA 损伤。Cisplatin 可激活铁死亡 (ferroptosis) 并诱导自噬 (autophagy)。