Asiatic acid, a pentacyclic triterpene from Centella asiatica, exhibits anticancer activities, induces apoptosis in melanoma cells, and provides barrier protective effects in human aortic endothelial cells (HAEC). It also demonstrates anti-inflammatory properties by inhibiting TNF-α-induced endothelial barrier dysfunction and the NLRP3 inflammasome activation along with the NF-κB pathway. These actions result in significant inflammation reduction in rats and offer neuroprotection in rat spinal cord injury models[1][2][3][4][5].
体内研究
Asiatic acid, administered via intragastric gavage at dosages ranging from 5 to 75 mg/kg once daily, has been found to effectively inhibit pelvic inflammatory disease (PID) in rats within a pathogen-induced disease model, demonstrating antioxidant and anti-inflammatory effects[3].
Asiatic acid, administered through intragastric injection at dosages of 30 mg/kg and 75 mg/kg once daily, exhibits neuroprotective effects on spinal cord injury (SCI) in rats by mitigating inflammation and oxidative stress[4].
体外研究
Asiatic acid stabilizes the barrier against TNF-α by preserving the structure of adherens junctions (AJs) and preventing the redistribution of tight junctions (TJs)[2].
Asiatic acid at a concentration of 40 μM maintains the stability of F-actin and diphosphate-MLC at the cellular periphery, thereby inhibiting their reorganization triggered by TNF-α (10 ng/mL for 1 hour). Conversely, Asiatic acid, within the range of 10-30 μM over 6 hours, does not diminish F-actin aggregation[2].
Asiatic acid, at a concentration of 40 μM administered for 6 hours, maintains the network structure of cell-cell contact regions and counteracts the TNF-α-induced structural rearrangement of vascular endothelial (VE)-cadherin and β-catenin[2].
Nonetheless, Asiatic acid, when applied in doses ranging from 10 to 40 μM for a duration of 6 hours, does not exhibit any inhibitory effect on the increased endothelial cell permeability caused by cytochalasin D (5 μM for 15 minutes). It has been observed that Asiatic acid enhances the diphosphorylation of myosin light chain (MLC)[2].
搜索
