Cycloleucine acts as a specific blocker of S-adenosyl-methionine dependent methylation processes and antagonizes the NMDA receptor-associated glycine receptor, evidenced by a Ki value of 600 μM. It also competitively inhibits ATP: L-methionine-S-adenosyl transferase in vitro, displaying anxiolytic and cytostatic effects[1].[2].[3].[4].
体内研究
Administered intracerebroventricularly at doses between 0.5 and 4 μg, cycloleucine enhances exploratory behavior in rats, as shown by increased time spent in open arms, more entries into open arms, and increased extreme arrivals during tests[3].
Additionally, cycloleucine leads to a reduction in the weights of the thymus and spleen in both infected and control mice exposed to the Semliki Forest virus (SFV) strain A7(74)[6].
体外研究
Treatment with cycloleucine, ranging from 4 to 40 mM for 3 hours, effectively prevents internal methylation of viral RNA in B77 transformed chick embryo fibroblasts[5].
At a concentration of 40 mM for 24 hours, cycloleucine significantly impedes the formation of m6A and the penultimate Gm in the B77 38S RNA subunits by over 90%[5].
Cytostatic, at a dose of 10 μg/mL, diminishes the viability of human KB and mouse L1210s leukemia cell lines[5].
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