tracheloside是一种天然产物,显著降低碱性磷酸酶(AP)活性,这是一种雌激素诱导的标志酶,其IC50值为0.31 mg/mL,与他莫昔芬(IC50 = 0.43 mg/mL)相当。
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描述 | Tracheloside is a lignan glycoside isolated from the seeds of Trachelospermum jasminoides with anti-estrogenic activity. It has been shown to decreased the activity of alkaline phosphatase in Ishiwaka cells with an IC50 value of 0.31μg/mL[3]. Treatment of HaCaT cells with tracheloside at 1, 5, and 10μg/mL increased cell proliferation rate by 13.98%, 18.82%, and 17.94%, respectively. Tracheloside treatment (1, 5, and 10μg/mL) also increased cell healing activity by 38.14%, 106.13%, and 72.83%, respectively, compared to the control group. Also in HaCaT cells, tracheloside treatment at 1, 5, and 10μg/mL led to 1.3-, 1.67-, and 2.73-fold increase in the phosphorylation of ERK1/2, respectively[4]. In Sprague-Dawley rats, diet supplementation with 0.2% or 0.02% tracheloside did not significantly affect PhIP (100mg/kg body weight)-induced mammary carcinogenesis[5]. |
计算器 | ||||
存储液制备 | ![]() |
1mg | 5mg | 10mg |
1 mM 5 mM 10 mM |
1.82mL 0.36mL 0.18mL |
9.08mL 1.82mL 0.91mL |
18.16mL 3.63mL 1.82mL |
CAS号 | 33464-71-0 |
分子式 | C27H34O12 |
分子量 | 550.552 |
别名 | |
运输 | 蓝冰 |
存储条件 |
In solvent -20°C:3-6个月-80°C:12个月 Pure form Inert atmosphere,Store in freezer, under -20°C |
溶解方案 |
DMSO: 105 mg/mL(190.72 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO 以下溶解方案都请先按照体外实验的方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议现用现配,当天使用; 以下溶剂前显示的百分比是指该溶剂在终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶
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动物实验配方 |