There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Deubiquitylating enzymes (DUBs) antagonize the ubiquitylation of substrates by cleaving mono and polyubiquitin and thus afford an additional level of post-translational regulation. USP7 (Ubiquitin-specific protease 7) is a deubiquitinase that has activity on numerous targets, including the E3 ligase MDM2[3]. P00591 is a selective inhibitor of USP7 and USP47 activity with IC50 values of 4.2 μM and 4.3 μM[4]. P005091 stabilizes p53, inhibits cancer cell proliferation, and is active in cellular models of disease. In vitro, P005091 accelerated the degradation of the USP7 substrate HDM2 in several cancer cell lines, inhibited the growth of HCT-116 human colorectal cancer cells with an IC50 of 11 μM, and inhibited cancer cell growth synergistically with doxorubicin, etoposide, or mechlorethamine[4]. In vivo, P00591 decreased expression of PCNA and triggered cleavage of PARP and caspase-3 in a dose-dependent manner. Administration of P00591 at 10 mg/kg leaded to enhanced proliferation inhibition and apoptosis in tumor tissues in mouse colon cancer CT26 cells xenograft mice. Treatment with 10 mg/kg P005091 promoted the CD4+ and CD9+ T-cell-mediated antitumor immune response, and inhibited FOXP3+ Treg function in CT26 cells xenograft mice[5].
P005091 细胞研究
细胞系
浓度
检测类型
检测时间
活动说明
数据源
human HCT116 cells
Cytotoxic assay
72 h
Cytotoxicity against human HCT116 cells after 72 hrs, EC50=11 μM
24900381
Sf9 cells
Function assay
Inhibition of recombinant USP7 expressed in Sf9 cells by Ub-CHOP reporter assay, EC50=4.2 μM
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