MPEP (1-30 mg/kg) elicits anxiolytic-like effects in various behavioral tests, including the conflict drinking test and the elevated plus-maze test in rats, as well as the four-plate test in mice^[2].

MPEP (1-20 mg/kg) reduces the immobility time in a tail suspension test in mice, though it shows no activity in the behavioral despair test in rats^[2].

MPEP (30 mg/kg i.p.) significantly increases the number of punished crossings by 39% in the four-plate test, whereas lower doses (3 and 10 mg/kg) do not affect punished crossings in that test (F (3,36)=3.240, P<0.05)^[2].

MPEP administered at 1, 10, and 20 mg/kg significantly reduces the immobility time in the tail suspension test in mice by up to 55% after the highest dose (F(3,28)=15.47, P<0.001), demonstrating efficacy comparable to that of imipramine (20 mg/kg), which is used as the positive control^[2].

MPEP does not exhibit agonistic or antagonistic effects at 100 mM on human mGlu2, -3, -4a, -7b, and -8a receptors, nor at 10 μM on the human mGlu6 receptor^[1].