GM 6001 is a broad spectrum MMP inhibitor with Ki values of 0.1nM, 0.2nM, 0.36nM, 0.4nM, 0.5nM, 3.6nM and 3.7nM for MMP-8, MMP-9, MMP-26, MMP-1, MMP-2, MMP-12 and MMP-7[1]. The tyrosine phosphorylation of EGFR induced by leptin could be significantly attenuated by treatment with 25μM GM6001 for 30min in rat aortic smooth muscle cells, due to the inhibition of proteolytic cleavage of transmembrane precursors via matrix metalloproteinase by GM6001[2]. Inhibition of MMP-2/MMP-9-mediated NCAM (Neural cell adhesion molecule) proteolysis by administration of 5μg GM6001 through intracerebroventricular route 30 minutes before and 2 h after ischemia attenuated the infarction, behavioral abnormalities of MCAO (middle cerebral artery occlusion) model mice, with suppression on the level of NCAM cleavage product by GM6001 observed[3].
Ilomastat 细胞研究
细胞系
浓度
检测类型
检测时间
活动说明
数据源
HEk293 cells
Function assay
Inhibition of human recombinant ADAMTS4 expressed in HEk293 cells using FAM-AEwLQGRPISIAK-TAMRA as substrate measured for 15 mins by fluorometric analysis, IC50=0.48 μM
23376997
human U251 cells
20 μM
Function assay
Protection against anthrax PA63-lethal toxin complex-induced cytotoxicity in human U251 cells at 20 uM at 400 ng/ml by MTT test
United States, New Mexico
... 展开 >>
Department of Neurology, University of New Mexico
Recruiting
Albuquerque, New Mexico, United States, 87131-0007
Contact: Asad Ikram, MD 505-272-9382 aikram@salud.unm.edu
Principal Investigator: Atif Zafar, MD
Sub-Investigator: Asad Ikram, MD 收起 <<
Inhibition of human recombinant ADAMTS4 expressed in HEk293 cells using FAM-AEwLQGRPISIAK-TAMRA as substrate measured for 15 mins by fluorometric analysis, IC50=0.48 μM
23376997
human U251 cells
20 μM
Function assay
Protection against anthrax PA63-lethal toxin complex-induced cytotoxicity in human U251 cells at 20 uM at 400 ng/ml by MTT test
United States, New Mexico
... 展开 >>
Department of Neurology, University of New Mexico
Recruiting
Albuquerque, New Mexico, United States, 87131-0007
Contact: Asad Ikram, MD 505-272-9382 aikram@salud.unm.edu
Principal Investigator: Atif Zafar, MD
Sub-Investigator: Asad Ikram, MD 收起 <<
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