The dual-specificity tyrosine-phosphorylation-regulated (DYRK) kinases belong to a family collectively referred to as CMGC kinases that includes cyclin dependent kinases, mitogen associated protein kinases, glycogen synthase kinases, and cyclin dependent-like kinases. ID-8, a DYRK inhibitor, shows high specificity for DYRK1A and DYRK1B with IC50s of 78 and 54 nM, respectively[3]. ID-8 increased hESC (human embryonic stem cells) survival (∼1.1% at 0.5 μM). The combination of Wnt3a and ID-8 further increased survival (∼1.7%) and completely prevented Wnt-induced differentiation morphologically without disrupting proliferation. Wnt-induced differentiation marker gene expression was dramatically reduced by ID-8 at a dose that maintained the undifferentiated state[4]. Treatment with ID-8 (10 µM) promoted significant increases in cell number after 24 hours of damage in at least two different damage models[5].
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