Moreover, Folinic acid treatment (7.0 mg/kg; intraperitoneal injection; every second day; for 3 weeks) in Balb/c young growing male mice reverses the growth inhibition caused by chronic MTX administration, specifically addressing the suppression of skeletal growth observed with MTX^[2].

It effectively decreases the percentage of micronucleated binucleated cells (% MNBN) and aberrant cells (% Abs), which are elevated by MTX. Additionally, Folinic acid at a concentration of 50 μg/mL notably reduces these percentages (40-68% for MNBN and 36-77% for Abs). Even at a lower concentration of 5 μg/mL, Folinic acid demonstrates a significant inhibitory effect (12 to 54% for MNBN and 20 to 61% for Abs). This indicates its efficacy in protecting cells from MTX-induced genetic damage ^[1].