Adenosine 5'-monophosphate monohydrate functions as an agonist for the adenosine A1 receptor[1]. In cell studies using RAW264.7 cells, Adenosine 5'-monophosphate monohydrate demonstrates minimal cytotoxicity at concentrations ranging from 25 to 400 μM. Notably, it significantly reduces mRNA levels of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in a dose-dependent manner, with the most substantial suppression observed at 400 μM. Additionally, it impedes the recruitment of the NF-κB p65 subunit to the promoters of TNF-α, IL-6, and IL-1β genes, thereby inhibiting their expression[2].
体内研究
In vivo studies with C57BL/6J mice treated with Adenosine 5'-monophosphate monohydrate (5′-AMP) show a significant increase in hepatic adenosine levels. In comparison to a control group treated with PBS, where the survival rates were 60% after 8 hours and 33.3% after 24 hours, the treatment group displayed survival rates of 100% at 8 hours and 93.3% at 24 hours. Furthermore, this treatment resulted in notably lower serum levels of aspartate transaminase (AST) and alanine transaminase (ALT), reduced liver necrosis, and decreased infiltration of inflammatory cells[2].
体外研究
Adenosine 5'-monophosphate monohydrate functions as an agonist for the adenosine A1 receptor[1].
In cell studies using RAW264.7 cells, Adenosine 5'-monophosphate monohydrate demonstrates minimal cytotoxicity at concentrations ranging from 25 to 400 μM. Notably, it significantly reduces mRNA levels of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in a dose-dependent manner, with the most substantial suppression observed at 400 μM. Additionally, it impedes the recruitment of the NF-κB p65 subunit to the promoters of TNF-α, IL-6, and IL-1β genes, thereby inhibiting their expression[2].
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