货号:A551904 同义名: 8-溴腺苷-3',5'-环单磷酸钠 / 8-Bromo-cAMP sodium salt;8-Br-Camp sodium salt
8-Bromo-cAMP is a cell permeable analog of cAMP that activates cyclic-AMP-dependent protein kinase with a Ka value of 0.05 μM and a PKA activator.
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产品名称 | PKA ↓ ↑ | 其他靶点 | 纯度 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Daphnetin |
+
PKA, IC50: 9.33 μM |
EGFR,PKC | 95% | ||||||||||||||||
AT13148 |
++++
PKA, IC50: 3 nM |
98+% | |||||||||||||||||
A-674563 HCl |
+++
PKA, Ki: 16 nM |
98% | |||||||||||||||||
H-89 2HCl |
++
PKA, Ki: 48 nM |
99%+ | |||||||||||||||||
1. 鼠标悬停在“+”上可以显示相关IC50的具体数值。"+"越多,抑制作用越强。2. "✔"表示该化合物对相应的亚型有抑制作用,但抑制强度暂时没有相关数据。 |
描述 | The cAMP/PKA signaling pathway regulates a wide range of cellular processes. 8-Bromo-cAMP is an activator of PKA with a Ka value of 0.05 μM[3]. Treatment of 0.1 and 0.5 mM 8-Bromo-cAMP significantly increased the number of NANOG positive ES cell-like colonies in human fibroblast cells compared to the negative control. The combination of 0.1 mM 8-Bromo-cAMP and 0.5 mM VPA inhibited the proliferation rates at day 3, 4 and 5 in HFF1 cells. The same co-treatment of 8-Bromo-cAMP and VPA in HFF1 cells also induced the upregulation of cytokine-related and inflammatory pathways at day 1, and down-regulated the p53, cell cycle and RB pathways[4]. In BEAS-2B cells, pretreatment of 100 μM 8-Bromo-cAMP for one hour significantly reduced HDE-induced release of IL-6 and IL-8 compared to cells only received 24-h treatment of 5% HDE with presence of 100 μM 8-Br-cAMP. Also in BEAS-2B cells, HDE (5%) significantly promoted the maximal PKC-ε activity at 6h, but pretreatment with 100 μM 8-Bromo-cAMP for hour blocked HDE-stimulated PKC-ε. The 1-h treatment with 8-Bromo-cAMP (0.1–10 μM) also significantly increased PKA activity in a dose-dependent manner[5]. In ddY mice, pretreatment with 8-Br-cAMP (i.p., 10 mg/kg) prior to LPS injection significantly inhibited LPS-induced dye leakage in the skin and the increase of serum TNF-α level[6]. |
作用机制 | 8-Bromo-cAMP is a brominated derivative of cAMP that functions as a cell-permeable analog of cAMP to activate cAMP-dependent protein kinase (PKA)[5]. |
Dose | Cat: 0.35 mg/kg - 1 mg/kg[3] (intra-arterial injection) Mice: 330 mg/kg[4] (i.p.), 10 mg/kg[5] (i.p.) |
Administration | Intra-arterial injection, i.p. |
计算器 | ||||
存储液制备 | 1mg | 5mg | 10mg | |
1 mM 5 mM 10 mM |
2.33mL 0.47mL 0.23mL |
11.63mL 2.33mL 1.16mL |
23.25mL 4.65mL 2.33mL |
CAS号 | 76939-46-3 |
分子式 | C10H10BrN5NaO6P |
分子量 | 430.084 |
别名 | 8-溴腺苷-3',5'-环单磷酸钠 ;8-Bromo-cAMP sodium salt;8-Br-Camp sodium salt;8 Br cAMP Na;8-Br-cAMP sodium;8-Bromoadenosine 3',5'-cyclic monophosphate;8-bromo-Cyclic AMP (sodium salt);8-Bromo-cAMP |
运输 | 蓝冰 |
存储条件 |
液体 -20°C:3-6个月-80°C:12个月 粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C |
溶解度 |
DMSO: 120 mg/mL(279.02 mM),注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO H2O: 100 mg/mL(232.51 mM),配合低频超声助溶 |
动物实验配方 |