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8-Bromoadenosine 3',5'-cyclic monophosphate sodium salt

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Chemical Structure| 76939-46-3 同义名 : 8-溴腺苷-3',5'-环单磷酸钠 ;8-Bromo-cAMP sodium salt;8-Br-Camp sodium salt;8 Br cAMP Na;8-Br-cAMP sodium;8-Bromoadenosine 3',5'-cyclic monophosphate;8-bromo-Cyclic AMP (sodium salt);8-Bromo-cAMP
CAS号 : 76939-46-3
货号 : A551904
分子式 : C10H10BrN5NaO6P
纯度 : 99%+
分子量 : 430.084
MDL号 : MFCD00005844
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(279.02 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 100 mg/mL(232.51 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 The cAMP/PKA signaling pathway regulates a wide range of cellular processes. 8-Bromo-cAMP is an activator of PKA with a Ka value of 0.05 μM[3]. Treatment of 0.1 and 0.5 mM 8-Bromo-cAMP significantly increased the number of NANOG positive ES cell-like colonies in human fibroblast cells compared to the negative control. The combination of 0.1 mM 8-Bromo-cAMP and 0.5 mM VPA inhibited the proliferation rates at day 3, 4 and 5 in HFF1 cells. The same co-treatment of 8-Bromo-cAMP and VPA in HFF1 cells also induced the upregulation of cytokine-related and inflammatory pathways at day 1, and down-regulated the p53, cell cycle and RB pathways[4]. In BEAS-2B cells, pretreatment of 100 μM 8-Bromo-cAMP for one hour significantly reduced HDE-induced release of IL-6 and IL-8 compared to cells only received 24-h treatment of 5% HDE with presence of 100 μM 8-Br-cAMP. Also in BEAS-2B cells, HDE (5%) significantly promoted the maximal PKC-ε activity at 6h, but pretreatment with 100 μM 8-Bromo-cAMP for hour blocked HDE-stimulated PKC-ε. The 1-h treatment with 8-Bromo-cAMP (0.1–10 μM) also significantly increased PKA activity in a dose-dependent manner[5]. In ddY mice, pretreatment with 8-Br-cAMP (i.p., 10 mg/kg) prior to LPS injection significantly inhibited LPS-induced dye leakage in the skin and the increase of serum TNF-α level[6].
作用机制 8-Bromo-cAMP is a brominated derivative of cAMP that functions as a cell-permeable analog of cAMP to activate cAMP-dependent protein kinase (PKA)[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.33mL

0.47mL

0.23mL

11.63mL

2.33mL

1.16mL

23.25mL

4.65mL

2.33mL
参考文献

[1]Wyatt TA, Poole JA, et al. cAMP-dependent protein kinase activation decreases cytokine release in bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2014 Oct 15;307(8):L643-51.

[2]Wang Y, Adjaye J. A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells. Stem Cell Rev. 2011 Jun;7(2):331-41.

[3]Sandberg M, Butt E, Nolte C, Fischer L, Halbrügge M, Beltman J, Jahnsen T, Genieser HG, Jastorff B, Walter U. Characterization of Sp-5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole- 3',5'-monophosphorothioate (Sp-5,6-DCl-cBiMPS) as a potent and specific activator of cyclic-AMP-dependent protein kinase in cell extracts and intact cells. Biochem J. 1991 Oct 15;279 ( Pt 2)(Pt 2):521-7.

[4]Wang Y, Adjaye J. A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells. Stem Cell Rev Rep. 2011 Jun;7(2):331-41.

[5]Wyatt TA, Poole JA, Nordgren TM, DeVasure JM, Heires AJ, Bailey KL, Romberger DJ. cAMP-dependent protein kinase activation decreases cytokine release in bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2014 Oct 15;307(8):L643-51.

[6]Irie K, Fujii E, Ishida H, Wada K, Suganuma T, Nishikori T, Yoshioka T, Muraki T. Inhibitory effects of cyclic AMP elevating agents on lipopolysaccharide (LPS)-induced microvascular permeability change in mouse skin. Br J Pharmacol. 2001 May;133(2):237-42.