7-Amino-4-methyl-3-coumarinylacetic acid (AMCA), a new fluorescent protein labeling agen, emits in the blue region (440-460 nm) on activation with UV light (350 nm). The active reagent is the N-hydroxysuccinimide ester which reacts with lysine residues under mild conditions to form photostable amide links. AMCA-immunoglobulin conjugates were not susceptible to photobleaching and have a storage life at -20 degrees C of more than two years[1]. Moreover, visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, AMCA, in combination with green and red fluorescing FITC and TRITC. Three-color in situ hybridization was applied to the study of numerical chromosome abnormalities as occur in human solid tumors[2]. Using E. coli as a scaffold, an amine coupling reaction was used to covalently attach glycine, spermine, bovine serum albumin (protein), or AMCA to the free carboxylic acid groups on the surface of the cells. These populations, along with unlabeled control cells, were subject to electrokinetic and dielectrophoretic measurements to quantify any changes in the biophysical properties upon alteration[3].
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