规格 | 价格 | 会员价 | 库存 | 数量 | |||
---|---|---|---|---|---|---|---|
{[ item.pr_size ]} |
{[ getRatePrice(item.pr_rmb, 1,1) ]} {[ getRatePrice(item.pr_rmb_sale, 1,1) ]} |
{[ getRatePrice(item.pr_rmb, 1,1) ]} {[ getRatePrice(item.pr_rmb,item.pr_rate,1) ]} |
{[ getRatePrice(item.pr_rmb, 1,1) ]} | {[ getRatePrice(item.pr_rmb_sale, 1,1) ]} {[ getRatePrice(item.pr_rmb,item.pr_rate,item.mem_rate) ]} {[ getRatePrice(item.pr_rmb,1,item.mem_rate) ]} | 现货 | 咨询 | - + |
快速发货 顺丰冷链运输,1-2 天到达
品质保证
技术支持
免费溶解
描述 | 4μ8C (IRE1 Inhibitor III) is a small-molecule inhibitor of IRE1α[1]. Additionally impedes Xbp1 mRNA splicing in a dose-responsive manner when added to the culture media of ER-stressed cells. This inhibitor's binding to IRE1 is characterized by a slow dissociation rate, although removal of the inhibitor allows for a quick resumption of Xbp1 splicing in the cells[1]. The mechanism by which 4μ8C operates, especially in preventing the splicing of XBP1 mRNA under ER stress conditions, such as those induced by mutant proinsulin production, provides insight into its potential application in managing ER stress-related cellular dysfunctions[2]. Treatment with 4μ8C significantly reduces IL-4 production by CD4+ T cells under Th0 conditions, as evidenced by decreased IL-4 levels in culture supernatants and a lower percentage of IL-4 positive cells. The production of IL-5 and IL-13 is also notably diminished following 4μ8C administration[3]. |
体内研究 | Intraperitoneal injection of 4μ8c (IRE1 Inhibitor III), at a dosage of 10 mg/kg/day for an additional four weeks, results in a marked decrease (45.2%) in the atherosclerotic lesion area in en face aorta samples, demonstrating 4μ8c's capacity to effectively impede plaque formation in mice[4]. Oral administration of 4μ8C at doses of 10, 50, or 100 mg/kg efficiently curtails passive cutaneous anaphylaxis (PCA) in mice, with an effective dose 50 (ED50) of 25.1 mg/kg[5]. Additionally, 4μ8C counteracts the ER stress-induced depletion of several recognized RIDD targets, showcasing an EC50 roughly equivalent to that for the inhibition of XBP1 target gene activation, approximately 4 μM[1]. |
体外研究 | 4μ8C (IRE1 Inhibitor III) is a small-molecule inhibitor of IRE1α[1]. Additionally impedes Xbp1 mRNA splicing in a dose-responsive manner when added to the culture media of ER-stressed cells. This inhibitor's binding to IRE1 is characterized by a slow dissociation rate, although removal of the inhibitor allows for a quick resumption of Xbp1 splicing in the cells[1]. The mechanism by which 4μ8C operates, especially in preventing the splicing of XBP1 mRNA under ER stress conditions, such as those induced by mutant proinsulin production, provides insight into its potential application in managing ER stress-related cellular dysfunctions[2]. Treatment with 4μ8C significantly reduces IL-4 production by CD4+ T cells under Th0 conditions, as evidenced by decreased IL-4 levels in culture supernatants and a lower percentage of IL-4 positive cells. The production of IL-5 and IL-13 is also notably diminished following 4μ8C administration[3]. |
细胞系 | 浓度 | 检测类型 | 检测时间 | 活动说明 | 数据源 |
human Jeko cells | Function assay | 24 h | Inhibition of XBP-1s expression in human Jeko cells after 24 hrs by immunoblotting analysis, IC50=1.57 μM | 24749861 | |
human Mino cells | Function assay | 24 h | Inhibition of XBP-1s expression in human Mino cells after 24 hrs by immunoblotting analysis, IC50=1.62 μM | 24749861 | |
SF21 cells | Function assay | 30 mins | Inhibition of human recombinant puritin-His-tagged IRE-1 RNase expressed in SF21 cells using XBP-1 RNA stem loop as substrate incubated for 30 mins prior to substrate addition measured after 2 hrs by FRET-suppression assay, IC50=0.206 μM | 24749861 |
计算器 | ||||
存储液制备 | ![]() |
1mg | 5mg | 10mg |
1 mM 5 mM 10 mM |
4.90mL 0.98mL 0.49mL |
24.49mL 4.90mL 2.45mL |
48.98mL 9.80mL 4.90mL |
CAS号 | 14003-96-4 |
分子式 | C11H8O4 |
分子量 | 204.179 |
别名 | IRE1 Inhibitor III |
运输 | 蓝冰 |
存储条件 |
粉末 Inert atmosphere,Store in freezer, under -20°C 液体 -20°C:3-6个月-80°C:12个月 |
溶解度 |
DMSO: 25 mg/mL(122.44 mM),注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO |
动物实验配方 |
5%DMSO+40%PEG300+5%Tween80+50%water 0.5 mg/mL |