There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Trypsin is a serine protease enzyme that cleaves proteins at the carboxyl side of lysine or arginine residues. It activates PAR2 and PAR4 receptors and facilitates cell-to-cell membrane fusion during PDCoV infection by interacting with the S glycoprotein of PDCoV and pAPN. Additionally, trypsin promotes cell proliferation and differentiation. It finds application in wound healing and the study of neurogenic inflammation[1][2][3][4][6]. Trypsin MS grade, a serine protease enzyme, cleaves proteins on the carboxyl end of Lysine or Arginine and activates PAR2 and PAR4. It facilitates cell-to-cell membrane fusion during PDCoV infection through the interaction between PDCoV's S glycoprotein and pAPN, and enhances cell proliferation and differentiation. This enzyme is applicable in studying wound healing and neurogenic inflammation [1][2][3][4][6]. Recombinant Trypsin Solution, an animal-free enzyme, is utilized for cell or tissue digestion. It offers high stability at room temperature and performs gentle digestion, making it suitable for processing weakly adherent cells or stem cells in low serum or serum-free conditions. Unlike conventional trypsin solutions, Recombinant Trypsin Solution is devoid of animal-derived components, providing a gentle yet effective alternative for cell digestion without using animal-derived trypsin [1].
体内研究
Trypsin (100-500 μg per site in 50 μL saline, intradermal injection) elicits scratching behavior in mice[5].
Trypsin MS grade (100-500 μg per site in 50 μL saline, intradermal injection) triggers scratching behavior in mice [5].
体外研究
Trypsin is a serine protease enzyme that cleaves proteins at the carboxyl side of lysine or arginine residues. It activates PAR2 and PAR4 receptors and facilitates cell-to-cell membrane fusion during PDCoV infection by interacting with the S glycoprotein of PDCoV and pAPN. Additionally, trypsin promotes cell proliferation and differentiation. It finds application in wound healing and the study of neurogenic inflammation[1][2][3][4][6].
Trypsin (10 and 50 ng/mL, 12 h) facilitates PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion[2].
Trypsin (0.05%, 3 h) stimulates the proliferation of C6 glioma cells in serum-free and growth factor-free medium[3].
Trypsin (20 -150 ng/mL, 5 days) enhances the differentiation of peripheral blood mononuclear cells (PBMCs)[4].
Trypsin MS grade (5 μg/mL, 24 or 48 h) enhances the replication of porcine deltacoronavirus (PDCoV) in LLC-PK cells [2].
Trypsin MS grade (10 and 50 ng/mL, 12 h) facilitates PDCoV cell-to-cell spread in LLC-PK cells by encouraging membrane fusion within these cells [2].
Trypsin MS grade (0.05%, 3 h) stimulates the proliferation of C6 glioma cells in a medium free of serum and growth factors [3].
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