SP-96 is a potent, selective, and non-ATP-competitive inhibitor of Aurora B (IC50=0.316 nM), exhibiting over 2000-fold selectivity against FLT3 (IC50=1475.6 nM) and KIT (IC50=1307.6 nM)^[1].

SP-96 (0-1 µM; 24 hours) exhibits selectivity rather than promiscuity towards specific cell lines. It inhibits the growth of MDA-MB-468, CCRF-CEM, COLO 205, and A498 cells with GI50 values of 107 nM, 47.4 nM, 50.3 nM, and 53.2 nM, respectively^[1].

SP-96 (63.2 nM) suppresses Aurora B activity in H460 cells, leading to increased DNA content and enlarged cell volume with enlarged nuclei^[1].

SP-96 (0-2 µM) suppresses Aurora B enzymatic activity with an IC50 of 0.316 nM and Aurora A with an observed IC50 value of 18.975 nM. It demonstrates over 2000-fold selectivity against FLT3 (IC50=1475.6 nM) and KIT (IC50=1307.6 nM). Additionally, it inhibits other receptor tyrosine kinases (RTKs) such as EGFR, RET, and HER2, with IC50 values ≥2 µM^[1].