Purmorphamine (GMP) refers to Purmorphamine produced following GMP guidelines. GMP small molecules serve effectively as auxiliary reagents for cell therapy manufacture. Purmorphamine acts as a smoothened/Smo receptor agonist with an EC50 of 1 μM[1].
体外研究
Purmorphamine (10, 20 μM) in conjunction with sirolimus significantly reduces cell numbers according to the MTT assay. Purmorphamine induces up-regulation of alkaline phosphatase activity and expression of RUNX-2 on day 14. Up-regulation of osteocalcin is detected at the 3 and 5 μM doses of purmorphamine on day 14 post-induction. However, matrix mineralization remains unchanged in the presence or absence of purmorphamine[1].
Purmorphamine induces phosphorylation of STAT3 in the mouse ES cell line ES14 and the mesenchymal stem cell line C3H10T1/2[2].
Purmorphamine enhances the expression of osteoblast phenotype markers - ALP activity and bone-like nodule formation - in human bone marrow mesenchymal cells[3].
Purmorphamine (GMP), at a concentration of 1.5 μM for a duration of 1 week, can generate embryoid bodies (EBs) from human iPS cells (hiPS2) and hESCs (HSF1)[1].
Purmorphamine (GMP) at a concentration of 1.5 μM converts hPSCs to NKX2-1 positive MGE cells[2].
During days 28-35, Purmorphamine (GMP) at a concentration of 2 μM promotes ventralization of brain spheroids during the differentiation process from hiPSCs[3].
Purmorphamine (GMP), at a concentration of 1 μM, in combination with retinoic acid (RA, 2 μM) facilitates hiPSC differentiation into functional neural tissue, as indicated by the expression of the neuronal marker β-tubulin III (βT-III)[4].
Between days 5 and 7, Purmorphamine (GMP) induces differentiation of hESCs into dopaminergic (DA) neurons[5].
Purmorphamine 细胞研究
细胞系
浓度
检测类型
检测时间
活动说明
数据源
HEK293T cells
5 μM
Function assay
4 h
Inhibition of BODIPY-cyclopamine binding to Smo C-terminal cytoplasmic domain expressed in HEK293T cells at 5 uM after 4 hrs by fluorescence microscopy
16408088
mouse C3H10T1/2 cells
Function assay
Induction of osteogenesis in mouse C3H10T1/2 cells assessed as induction of osteoblast specific marker alkaline phosphatase by immunofluorescence method, EC50=1 μM
16408003
mouse Shh Light2 cells
Function assay
30 h
Activation of Shh in mouse Shh Light2 cells after 30 hrs by luciferase reporter gene assay, EC50=1 μM
Inhibition of BODIPY-cyclopamine binding to Smo C-terminal cytoplasmic domain expressed in HEK293T cells at 5 uM after 4 hrs by fluorescence microscopy
16408088
mouse C3H10T1/2 cells
Function assay
Induction of osteogenesis in mouse C3H10T1/2 cells assessed as induction of osteoblast specific marker alkaline phosphatase by immunofluorescence method, EC50=1 μM
16408003
mouse Shh Light2 cells
Function assay
30 h
Activation of Shh in mouse Shh Light2 cells after 30 hrs by luciferase reporter gene assay, EC50=1 μM
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