产品说明书

Purmorphamine

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Chemical Structure| 483367-10-8 同义名 : Shh Signaling Antagonist VI
CAS号 : 483367-10-8
货号 : A124253
分子式 : C31H32N6O2
纯度 : 98%
分子量 : 520.625
MDL号 : MFCD09037557
存储条件：

粉末 Keep in dark place,Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 35 mg/mL(67.23 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

IP 2% DMSO+2% Tween80+30% PEG300+water 1 mg/mL clear

PO 0.5% CMC-Na 40 mg/mL suspension

生物活性
描述 Purmorphamine acts as a smoothened/Smo receptor agonist with an EC50 of 1 μM[1].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
HEK293T cells 5 μM Function assay 4 h Inhibition of BODIPY-cyclopamine binding to Smo C-terminal cytoplasmic domain expressed in HEK293T cells at 5 uM after 4 hrs by fluorescence microscopy 16408088
mouse C3H10T1/2 cells Function assay Induction of osteogenesis in mouse C3H10T1/2 cells assessed as induction of osteoblast specific marker alkaline phosphatase by immunofluorescence method, EC50=1 μM 16408003
mouse Shh Light2 cells Function assay 30 h Activation of Shh in mouse Shh Light2 cells after 30 hrs by luciferase reporter gene assay, EC50=1 μM 16408088
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.92mL

0.38mL

0.19mL

9.60mL

1.92mL

0.96mL

19.21mL

3.84mL

1.92mL
参考文献

[1]Beloti MM, et al. Purmorphamine enhances osteogenic activity of human osteoblasts derived from bone marrow mesenchymal cells. Cell Biol Int. 2005 Jul;29(7):537-41.. . . .

[2]Gu D, et al. A role for transcription factor STAT3 signaling in oncogene smoothened-driven carcinogenesis. J Biol Chem. 2012 Nov 2;287(45):38356-66.

[3]Starenki D, et al. Selective Mitochondrial Uptake of MKT-077 Can Suppress Medullary Thyroid Carcinoma Cell Survival In Vitro and In Vivo. Endocrinol Metab (Seoul). 2015 Dec;30(4):593-603.

[4]Sharma R, et al. 3D Bioprinting Pluripotent Stem Cell Derived Neural Tissues Using a Novel Fibrin Bioink Containing Drug Releasing Microspheres. Front Bioeng Biotechnol. 2020 Feb 11;8:57.