Additionally, ML346 has been shown to prevent the aggregation of polyQ35 in a C. elegans model, indicating its potential effectiveness in altering protein aggregation and the toxicity associated with it^[1].

With an EC50 of 4600 nM in HeLa cells, ML346 stands out as a potent activator of Hsp70. At a concentration of 10 μM, ML346 is capable of restoring proteostasis, enhancing CFTR-mediated iodide conductance, and improving the proper folding of proteins located in two distinct cellular compartments^[1].

ML346 triggers a variety of responses, prominently inducing Hsp70, oxidative stress response genes such as HO1 and GCLM, and a 2.5-fold increase in BiP expression in WT MEF cells. Ranging from 0.5 to 25 μM, ML346 provides cytoprotective effects to cells following a severe 35-minute heat shock and offers two-fold protection against apoptosis induced by H2O2^[2].