CFDA-SE | 5(6)-Carboxyfluorescein diacetate succinimidyl ester;5(6)-CFDA N-succinmidyl ester;6-CFDA N-succinimidyl ester;150206-15-8;Carboxyfluorescein succinimidyl ester;CFSE | 5(6)-羧基二乙酸荧光素琥珀酰亚胺酯 | PAK

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5(6)-羧基二乙酸荧光素琥珀酰亚胺酯(CFDASE ) /CFDA-SE {[allProObj[0].p_purity_real_show]}

货号：A1148024 同义名： 5(6)-羧基二乙酸荧光素琥珀酰亚胺酯 / 5(6)-Carboxyfluorescein diacetate succinimidyl ester;5(6)-CFDA N-succinmidyl ester

CFDA-SE 是一种细胞示踪染料，通过与细胞内酯酶反应生成荧光产物。CFDA-SE 主要用于细胞增殖、迁移和追踪研究。

CFDA-SE 化学结构
CAS号：150347-59-4

CFDA-SE 3D分子结构
CAS号：150347-59-4

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CFDA-SE 纯度/质量文件产品仅供科研

货号：A1148024 标准纯度： {[allProObj[0].p_purity_real_show]} 产品说明书

批次查询：批次纯度: COA SDS NMR HPLC CNMR LC-MS

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PAK 亚型比较

产品名称	PAK ↓ ↑	PAK1 ↓ ↑	PAK2 ↓ ↑	PAK3 ↓ ↑	PAK4 ↓ ↑	PAK5 ↓ ↑	PAK6 ↓ ↑	纯度
IPA-3	+ PAK1, IC50: 2.5 μM	+ PAK1, IC50: 2.5 μM						{[allProObj[0].p_purity_real_show]}
FRAX486	+++ PAK4, IC50: 39 nM PAK1, IC50: 14 nM	+++ PAK1, IC50: 14 nM	++ PAK2, IC50: 33 nM	++ PAK3, IC50: 39 nM	+ PAK4, IC50: 575 nM			{[allProObj[0].p_purity_real_show]}
FRAX1036	++ PAK2, Ki: 72.4 nM PAK4, Ki: 23.3 nM	++ PAK1, Ki: 23.3 nM	++ PAK2, Ki: 72.4 nM		+ PAK4, Ki: 2.4 μM			{[allProObj[0].p_purity_real_show]}
FRAX597	++++ PAK2, IC50: 13 nM PAK3, IC50: 13 nM	++++ PAK1, IC50: 8 nM	++++ PAK2, IC50: 13 nM	+++ PAK3, IC50: 19 nM				{[allProObj[0].p_purity_real_show]}
PF-3758309	++++ PAK6, Ki: 17.1 nM PAK3, IC50: 190 nM	++++ PAK1, Ki: 13.7 nM	+ PAK2, IC50: 190 nM	++ PAK3, IC50: 99 nM	+++ PAK4, Ki: 18.7 nM	+++ PAK5, Ki: 18.1 nM	+++ PAK6, Ki: 17.1 nM	{[allProObj[0].p_purity_real_show]}
1. 鼠标悬停在“+”上可以显示相关IC50的具体数值。"+"越多，抑制作用越强。2. "✔"表示该化合物对相应的亚型有抑制作用，但抑制强度暂时没有相关数据。

CFDA-SE 生物活性

描述

CFDA-SE is a fluorescent dye capable of cell membrane penetration. It reacts with free amine groups in the cytoskeleton proteins inside the cell to form a fluorescent protein complex. Once inside the cell, CFDA-SE localizes to the cell membrane, cytoplasm, and nucleus, with the most intense fluorescence staining present in the nucleus^[1]. The CFDA-SE dye is consistently distributed to progeny during cell division and proliferation, with its dilution being proportional to the number of cell divisions. This dilution pattern can be monitored and quantified via flow cytometry using an excitation light of 488 nm, serving as a method for assessing cell proliferation^[1].

CFDA-SE 参考文献

[1]Banks HT, et al. Quantifying CFSE Label Decay in Flow Cytometry Data. Appl Math Lett. 2013 May 1;26(5):571-577.

CFDA-SE 实验方案

计算器

存储液制备

1mg

5mg

10mg

1 mM

5 mM

10 mM

1.79mL

0.36mL

0.18mL

8.97mL

1.79mL

0.90mL

17.94mL

3.59mL

1.79mL

CFDA-SE 技术信息

CAS号	150347-59-4
分子式	C₂₉H₁₉NO₁₁
分子量	557.46

别名	5(6)-羧基二乙酸荧光素琥珀酰亚胺酯 ;5(6)-Carboxyfluorescein diacetate succinimidyl ester;5(6)-CFDA N-succinmidyl ester;6-CFDA N-succinimidyl ester;150206-15-8;Carboxyfluorescein succinimidyl ester;CFSE
运输	蓝冰

存储条件

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月

溶解度

动物实验配方

5(6)-羧基二乙酸荧光素琥珀酰亚胺酯(CFDASE ) /CFDA-SE {[allProObj[0].p_purity_real_show]}

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