Acetylcysteine is a thiol-containing antioxidant that has been shown to inhibit the binding activity of NF-κB in response to multiple agonists in various cell types. Preincubation of human umbilical vein endothelial cells with acetylcysteine (0 – 40mM) for 1h dose-dependently inhibited IL-1-induced monocytic cell adhesion. The IC50 value of acetylcysteine was 20mM, and the 95% inhibition was observed at 40mM. Acetylcysteine treatment also resulted in a reduction in surface expression of E-selectin and VCAM-1 of 56% and 70%, respectively, in endothelial cells[4]. In addition, acetylcysteine promoted long-term survival of serum-deprived PC12 cells with an ED50 value of around 30nM. It dose-dependently prevented internucleosomal DNA cleavage at the doses of 10 – 80mM. The presence of acetylcysteine also promoted the survival of neonatal rat sympathetic neurons. In PC12 cells, the thymidine incorporation and proliferation were almost completely blocked by 60mM acetylcysteine with an ED50 value of approximately 30mM[5]. Administration of SAMP8 mice with acetylcysteine (100mg/kg, once daily, s.c.) for four weeks improved their cognition in both the T‐maze footshock avoidance paradigm and the lever press appetitive task when compared to mice treated with saline[6].
作用机制
Acetylcysteine is a thiol and mucolytic agent that acts as an antioxidant by maintaining the balance of intracellular glutathione. The free sulfhydryl group allows acetylcysteine to reduce disulphide bonds, thereby decreasing mucus viscosity and facilitating mucociliary clearance[7].
Acetylcysteine 细胞研究
细胞系
浓度
检测类型
检测时间
活动说明
数据源
HUVEC cells
5 mM
Function assay
4 h
Antioxidant activity in HUVEC cells assessed as reduction in H2O2-induced GSH activity at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs
22841280
HUVEC cells
5 mM
Function assay
4 h
Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as cell viability at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by MTT assay
22841280
rat PC12 cells
Function assay
24 h
Inhibition of hydrogen peroxide induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level
Antioxidant activity in HUVEC cells assessed as reduction in H2O2-induced GSH activity at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs
22841280
HUVEC cells
5 mM
Function assay
4 h
Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as cell viability at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by MTT assay
22841280
rat PC12 cells
Function assay
24 h
Inhibition of hydrogen peroxide induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level
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