产品说明书

Acetylcysteine

Print
Chemical Structure| 616-91-1 同义名 : 乙酰半胱氨酸;易咳净;N-乙酰-L-半胱氨酸 ;NAC;N-Acetylcysteine;N-Acetyl-L-cysteine
CAS号 : 616-91-1
货号 : A233740
分子式 : C5H9NO3S
纯度 : 98%
分子量 : 163.195
MDL号 : MFCD00004880
存储条件：

粉末 Keep in dark place,Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(643.4 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 100 mg/mL(612.76 mM)，配合低频超声助溶
动物实验配方：
生物活性
靶点

  • TNF-α
描述 Acetylcysteine is a thiol-containing antioxidant that has been shown to inhibit the binding activity of NF-κB in response to multiple agonists in various cell types. Preincubation of human umbilical vein endothelial cells with acetylcysteine (0 – 40mM) for 1h dose-dependently inhibited IL-1-induced monocytic cell adhesion. The IC50 value of acetylcysteine was 20mM, and the 95% inhibition was observed at 40mM. Acetylcysteine treatment also resulted in a reduction in surface expression of E-selectin and VCAM-1 of 56% and 70%, respectively, in endothelial cells[4]. In addition, acetylcysteine promoted long-term survival of serum-deprived PC12 cells with an ED50 value of around 30nM. It dose-dependently prevented internucleosomal DNA cleavage at the doses of 10 – 80mM. The presence of acetylcysteine also promoted the survival of neonatal rat sympathetic neurons. In PC12 cells, the thymidine incorporation and proliferation were almost completely blocked by 60mM acetylcysteine with an ED50 value of approximately 30mM[5]. Administration of SAMP8 mice with acetylcysteine (100mg/kg, once daily, s.c.) for four weeks improved their cognition in both the T‐maze footshock avoidance paradigm and the lever press appetitive task when compared to mice treated with saline[6].
作用机制 Acetylcysteine is a thiol and mucolytic agent that acts as an antioxidant by maintaining the balance of intracellular glutathione. The free sulfhydryl group allows acetylcysteine to reduce disulphide bonds, thereby decreasing mucus viscosity and facilitating mucociliary clearance[7].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
HUVEC cells 5 mM Function assay 4 h Antioxidant activity in HUVEC cells assessed as reduction in H2O2-induced GSH activity at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs 22841280
HUVEC cells 5 mM Function assay 4 h Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as cell viability at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by MTT assay 22841280
rat PC12 cells Function assay 24 h Inhibition of hydrogen peroxide induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level 17158454
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

6.13mL

1.23mL

0.61mL

30.64mL

6.13mL

3.06mL

61.28mL

12.26mL

6.13mL
参考文献

[1]Alvirez-Freites EJ, Carter JL, Cynamon MH. In vitro and in vivo activities of gatifloxacin against Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2002 Apr;46(4):1022-5.

[2]Tsai JC, Jain M, et al. Induction of apoptosis by pyrrolidinedithiocarbamate and N-acetylcysteine in vascular smooth muscle cells. J Biol Chem. 1996 Feb 16;271(7):3667-70.

[3]ACETYLCYSTEINE

[4]Faruqi RM, Poptic EJ, Faruqi TR, De La Motte C, DiCorleto PE. Distinct mechanisms for N-acetylcysteine inhibition of cytokine-induced E-selectin and VCAM-1 expression. Am J Physiol. 1997 Aug;273(2 Pt 2):H817-26.

[5]Ferrari G, Yan CY, Greene LA. N-acetylcysteine (D- and L-stereoisomers) prevents apoptotic death of neuronal cells. J Neurosci. 1995 Apr;15(4):2857-66. doi: 10.1523/JNEUROSCI.15-04-02857.1995.

[6]Farr SA, Poon HF, Dogrukol-Ak D, Drake J, Banks WA, Eyerman E, Butterfield DA, Morley JE. The antioxidants alpha-lipoic acid and N-acetylcysteine reverse memory impairment and brain oxidative stress in aged SAMP8 mice. J Neurochem. 2003 Mar;84(5):1173-83.

[7]Santus P, Corsico A, Solidoro P, Braido F, Di Marco F, Scichilone N. Oxidative stress and respiratory system: pharmacological and clinical reappraisal of N-acetylcysteine. COPD. 2014 Dec;11(6):705-17.