AM251, at a dosage of 3 mg/kg administered intraperitoneally, significantly decreases capsaicin-evoked nocifensive behavior in a genotype-dependent manner. AM251 (3 mg/kg, i.p.) reduces the duration of heat hypersensitivity in FAAH KO but not WT mice. AM251 suppresses capsaicin-evoked heat hypersensitivity in a time-dependent manner in FAAH KO but not in WT mice. Post-hoc analysis reveals that FAAH KO mice receiving vehicle (i.p.) display heightened thermal hypersensitivity at 30, 60, and 90 minutes post-capsaicin in comparison to FAAH KO animals receiving AM251)^[4].

Furthermore, AM251 influences anxiety-related behaviors in rats, as indicated by its impact on open arm entries and time spent in the open arms of an elevated plus maze. Significant reductions in these measures were noted at doses of 1 mg/kg and 5 mg/kg, suggesting anxiogenic effects at these concentrations^[5].

AM251 shows an agonist response in HEK293 cells, similar to that found in the yeast expression system^[2].

In macrophages, both unstimulated and stimulated by acetylated LDL, AM251 reduces cholesteryl ester synthesis. This effect is observed regardless of the presence or absence of CB2 receptors, indicating a broader scope of action beyond just cannabinoid receptor interaction^[3].