RS-1 increases the DNA binding activity of RAD51. It can also act as an enhancer of homology-directed repair and CRISPR/Cas9 mediated knock-in efficiency.
RAD51 filament formation is a key step in homologous recombination repair. RS-1 is a small molecule that enhances RAD51 binding and filament stability. RS-1 at a concentration of 0, 1–25μM stimulated strand assimilation activity of RAD51. RS-1 at 7.5μM promoted resistance of human cells to cross-linking chemotherapy[3]. Treatment of embryos with 7.5μM of RS-1 resulted in a significantly higher knock-in rate compared with the control group (26.1% vs. 4.4%). RS-1 at a dose of 7.5 and 15 μM also improved TALEN- and Cas9-mediated knock-in efficiency in rabbit embryos[4].
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