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RS-1

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Chemical Structure| 312756-74-4 同义名 : -
CAS号 : 312756-74-4
货号 : A122463
分子式 : C20H16Br2N2O3S
纯度 : 99%+
分子量 : 524.226
MDL号 : MFCD00348720
存储条件：

粉末 Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(200.3 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 RAD51 filament formation is a key step in homologous recombination repair. RS-1 is a small molecule that enhances RAD51 binding and filament stability. RS-1 at a concentration of 0, 1–25μM stimulated strand assimilation activity of RAD51. RS-1 at 7.5μM promoted resistance of human cells to cross-linking chemotherapy[3]. Treatment of embryos with 7.5μM of RS-1 resulted in a significantly higher knock-in rate compared with the control group (26.1% vs. 4.4%). RS-1 at a dose of 7.5 and 15 μM also improved TALEN- and Cas9-mediated knock-in efficiency in rabbit embryos[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.91mL

0.38mL

0.19mL

9.54mL

1.91mL

0.95mL

19.08mL

3.82mL

1.91mL
参考文献

[1]Song J, Yang D, et al. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat Commun. 2016 Jan 28;7:10548.

[2]Mason JM, Logan HL, et al. The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors. Cancer Res. 2014;74(13):3546-55.

[3]Jayathilaka K, Sheridan SD, Bold TD, et al. A chemical compound that stimulates the human homologous recombination protein RAD51. Proc Natl Acad Sci U S A. 2008;105(41):15848-15853. doi:10.1073/pnas.0808046105

[4]Song J, Yang D, Xu J, Zhu T, Chen YE, Zhang J. RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency. Nat Commun. 2016;7:10548. Published 2016 Jan 28. doi:10.1038/ncomms10548