Nociceptin, a heptadecapeptide, is the endogenous ligand of the nociceptin receptor, acting as a potent anti-analgesic. Nociceptin (1 μg/mL) significantly prevents LPS (10 ng/mL)-stimulated cell migration whereas it is ineffective when added alone. Nociceptin (1 nM-10 μM) elicits a concentration-dependent blockade of LPS-mediated cell migration, with a maximal effect at 1 and 10 μM. Nociceptin counteracts LPS-induced elevation of IL-1β mRNA levels. Nociceptin (1 μM) and NNC 55-0396 induce apoptotic cell death in U87 cells. Nociceptin (1 μM) counteracts LPS-induced [Ca2+]i increase in U87 cells via β-arrestin 2. Nociceptin counteracts the LPS-induced phosphorylation of PKC and ERK in U87 cells. Nociceptin inhibits the LPS-mediated transcriptional activation of NF-kB and AP-1 reporter genes[1]. The i.v. administration of nociceptin (10-100 nmol/kg) inhibits the micturition reflex in a naloxone-resistant manner. Intrathecal nociceptin (10 nmol/rat) produces urodynamic modifications similar to those induced by the i.v. administration. Intracerebroventricular (i.c.v.) administration of nociceptin (0.3-1 nmol/rat) also inhibited the micturition reflex in a naloxone-resistant manner suggesting a direct effect on supraspinal sites controlling the micturition. The application of nociceptin (5-50 nmol/rat) onto the bladder serosa when the intravesical volume was subthreshold for the triggering of the micturition reflex, activated the reflex in a dose-dependent manner; the same treatment produced a biphasic effect on the ongoing reflex[2]. Injection of nociceptin into mice stimulated Rec8 phosphorylation and meiotic chromosome dynamics in testes, whereas injection of nocistatin, a specific inhibitor for nociceptin, abolished them[3].
United States, California
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United States
Cerritos, California, United States, 90703
United States
Garden Grove, California, United States, 92845
United States, Florida
United States, Florida
Jacksonville, Florida, United States, 32256
United States
Lauderhill, Florida, United States, 33319
United States, Florida
Orlando, Florida, United States, 32801
United States, Illinois
United States
Libertyville, Illinois, United States, 60048
United States, New York
United States
Rochester, New York, United States, 14618
United States, Tennessee
United States
Memphis, Tennessee, United States, 38119 收起 <<
United States, California
... 展开 >>
United States
Cerritos, California, United States, 90703
United States
Garden Grove, California, United States, 92845
United States, Florida
United States, Florida
Jacksonville, Florida, United States, 32256
United States
Lauderhill, Florida, United States, 33319
United States, Florida
Orlando, Florida, United States, 32801
United States, Illinois
United States
Libertyville, Illinois, United States, 60048
United States, New York
United States
Rochester, New York, United States, 14618
United States, Tennessee
United States
Memphis, Tennessee, United States, 38119 收起 <<
United Kingdom
... 展开 >> Leicester Royal Infirmary
Recruiting
Leicester, Leicestershire, United Kingdom, LE1 5WW
Contact: Christopher P Hebbes, BSc cph16@leicester.ac.uk
University of Leicester
Recruiting
Leicester, Leicestershire, United Kingdom, LE2 7LX
Contact: Christopher P Hebbes, BSc cph16@leicester.ac.uk 收起 <<
Canada, Ontario
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Toronto, Ontario, Canada 收起 <<
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