Mitoquinone mesylate, a TPP-derived antioxidant, is specifically designed to target mitochondria for protection against oxidative stress[1]. Mitoquinone, also known as MitoQ, is a mitochondria-focused antioxidant. Optimal dosages for MitoQ and DecylTPP are determined from dose-response trials during 4-hour cold storage. Mitoquinone's protective effects against cold storage damage are initially assessed using MitoSOX Red, a dye that detects mitochondrial superoxide production. NRK cells under cold storage exhibit approximately a two-fold increase in fluorescence, indicating mitochondrial superoxide generation, compared to untreated cells. Mitoquinone significantly shields against cold storage-induced mitochondrial superoxide production, unlike DecylTPP, which fails to provide protection. Mitoquinone treatment substantially lowers mitochondrial superoxide generation, while kidneys treated with DecylTPP show similar mitochondrial superoxide levels to those subjected to cold storage alone[1].
Mitoquinone mesylate/米托醌甲磺酸盐 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Platelets
5 μM
10 minutes
To evaluate TRAP-6-induced platelet aggregation and P-selectin expression. Results showed that TRAP-6 (5 μM) significantly increased platelet aggregation (88% ± 4.74%), while MitoQ at 5 μM significantly reduced aggregation (58.3% ± 5.1%). TRAP-6-induced P-selectin expression was not affected by MitoQ.
Int J Mol Sci. 2020 Aug 27;21(17):6192.
3T3-L1 adipocytes
100 nM
30 min
MitoQ attenuated malonic acid-induced Fgf21, UCP1 and Cidea gene upregulation, suggesting that malonic acid-induced upregulation of FGF21 signalling is partly dependent on malonate altering mitochondrial redox signalling.
Nat Commun. 2025 Jan 2;16(1):170.
A549 cells
1 μM
24 h
To detect the effect of MitoQ on DEP-induced ROS levels, results showed that MitoQ did not significantly reduce ROS levels.
Environ Pollut. 2022 Jul 15;305:119292.
Normal rat kidney (NRK) cells
100 nM
48 h
MitoQ scavenged mitochondrial superoxide and prevented the increased protein expression of PGC1α and CORE II following MnSOD knockdown.
Redox Biol. 2014 Jan 23;2:348-57.
hASCs
1 µM
48 h
Mitoquinone was used to reduce mitochondrial reactive oxygen species (mtROS) and evaluate its effect on IDO activity. Results showed that Mitoquinone significantly reduced the response of P5 hASCs to IFN-γ stimulation, but had a lesser effect on P12 hASCs.
Front Immunol. 2021 Mar 10;12:621744.
MLE12 cells
500 nM
30 min
To evaluate the role of Mitoquinone in CdCl2-induced pyroptosis in MLE12 cells, the results showed that Mitoquinone significantly reduced CdCl2-induced mitochondrial ROS and the expression of pyroptosis-related proteins.
Cell Commun Signal. 2024 Nov 26;22(1):566.
Normal rat kidney (NRK cells)
100 nM
48 h
Mitoquinone prevented the increase in mitochondrial superoxide and nitrotyrosine following MnSOD knockdown by scavenging mitochondrial superoxide.
Redox Biol. 2014 Jan 23;2:348-57.
Mitoquinone mesylate/米托醌甲磺酸盐 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
CdCl2-induced lung injury model
Intraperitoneal injection
3 mg/kg
Once daily for 7 days
To evaluate CdCl2-induced lung injury and alveolar epithelial cell pyroptosis, the results showed that CdCl2 exposure significantly increased the lung tissue inflammation score and the expression of pyroptosis-related proteins.
Cell Commun Signal. 2024 Nov 26;22(1):566.
Rats
Cardiac metabolism model
Oral
500 μM
Daily for 2 weeks
MitoQ treatment improved cardiac energetics during Na elevation, reduced lactate and alanine concentrations, and reversed the shift in redox balance.
Nat Commun. 2024 May 20;15(1):4277
Rats
Maternal psychosocial stress model during pregnancy
Intravenous injection
90 μg/kg
Single injection, lasting 5 days
To investigate the effects of MitoQ-NP on placental oxidative stress induced by maternal psychosocial stress and its impact on offspring behavior and neurobiology. Results showed that MitoQ-NP treatment reduced placental and maternal oxidative stress and prevented anxiety-like behavior and neurobiological changes in male offspring induced by prenatal stress.
Neurobiol Stress. 2020 Nov 29;13:100281
Mitoquinone mesylate/米托醌甲磺酸盐 动物研究
Animal study
Mitoquinone administration markedly mitigates pancreatic edema and neutrophil infiltration. It also causes a dose-dependent rise in serum amylase, nearly doubling at the higher dosage. MitoQ treatment almost doubles lung MPO activity triggered by Caerulein, with a notable increase in serum IL-6 levels at a dosage of 10 mg/kg[2].
搜索
