货号:A752777 同义名: 溴化乙锭 / EtBr;Homidium bromide
Ethidium Bromide (EtBr) is sometimes added to running buffer during the separation of DNA fragments by agarose gel electrophoresis.
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Type | HazMat fee for 500 gram (Estimated) |
Excepted Quantity | USD 0.00 |
Limited Quantity | USD 15-60 |
Inaccessible (Haz class 6.1), Domestic | USD 80+ |
Inaccessible (Haz class 6.1), International | USD 150+ |
Accessible (Haz class 3, 4, 5 or 8), Domestic | USD 100+ |
Accessible (Haz class 3, 4, 5 or 8), International | USD 200+ |
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描述 | Ethidium Bromide (EtBr) is sometimes added to running buffer during the separation of DNA fragments by agarose gel electrophoresis. It is used because upon binding of the molecule to the DNA and illumination with a UV light source, the DNA banding pattern can be visualized. The mode of binding of EtBr is intercalation between the base pairs. This binding changes the charge, weight, conformation, and flexibility of the DNA molecule. The mobility of DNA was always less in the gels with EtBr. |
NCT号 | 适应症或疾病 | 临床期 | 招募状态 | 预计完成时间 | 地点 |
NCT02520076 | Type 1 Diabetes Mellitus | Phase 1 Phase 2 | Active, not recruiting | March 2020 | Canada, Alberta ... 展开 >> University of Alberta Edmonton, Alberta, Canada, T6G 2C8 收起 << |
NCT03536611 | Atrial Fibrillation ... 展开 >> Coronary Artery Disease 收起 << | Phase 4 | Not yet recruiting | June 30, 2020 | - |
NCT02853006 | Lung Cancer | Not Applicable | Recruiting | June 2019 | France ... 展开 >> UniversityHospitalGrenoble Recruiting La Tronche, France, 38700 Contact: Christian Brambilla, Professor +33686793466 CBrambilla@chu-grenoble.fr Contact: Hélène Sabbah-Guillaume 04 76 76 68 16 HSabbahguillaume@chu-grenoble.fr 收起 << |
计算器 | ||||
存储液制备 | 1mg | 5mg | 10mg | |
1 mM 5 mM 10 mM |
2.54mL 0.51mL 0.25mL |
12.68mL 2.54mL 1.27mL |
25.36mL 5.07mL 2.54mL |
CAS号 | 1239-45-8 |
分子式 | C21H20BrN3 |
分子量 | 394.308 |
别名 | 溴化乙锭 ;EtBr;Homidium bromide |
运输 | 蓝冰 |
存储条件 |
In solvent -20°C:3-6个月-80°C:12个月 Pure form Keep in dark place,Inert atmosphere,Room temperature |
溶解方案 |
DMSO: 25 mg/mL(63.4 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO 以下溶解方案都请先按照体外实验的方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议现用现配,当天使用; 以下溶剂前显示的百分比是指该溶剂在终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶
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动物实验配方 |