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台盼蓝 /Diphenyl Blue {[allProObj[0].p_purity_real_show]}

货号:A621935 同义名: 曲利苯蓝 / Direct Blue 14;Trypan Blue

Diphenyl Blue is used as reference dye for group of azo dye.

HazMat Fee +

There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.

Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Diphenyl Blue 化学结构 CAS号:72-57-1
Diphenyl Blue 化学结构
CAS号:72-57-1
Diphenyl Blue 3D分子结构
CAS号:72-57-1
Diphenyl Blue 化学结构 CAS号:72-57-1
Diphenyl Blue 3D分子结构 CAS号:72-57-1
规格 价格 会员价 库存 数量
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Diphenyl Blue 纯度/质量文件 产品仅供科研

货号:A621935 标准纯度: {[allProObj[0].p_purity_real_show]}
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Diphenyl Blue 生物活性

描述 Trypan Blue is an acid azo dye commonly used as a stain to distinguish viable from non-viable cells. It is a vital stain used intra operatively during cataract surgery to stain the external surface of the anterior lens capsule for better visualization. Direct staining of the internal surface of anterior capsule with trypan blue affected LECs and the basement membrane. Treating the internal surface of capsular bag with trypan blue may reduce incidence of Posterior capsular opacification[1]. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells)[2]. In viable cells trypan blue-labelled vesicles indicate trypan blue uptake by macropinocytosis and trypan blue-labelled cytosol could indicate a further entry pathway for the dye, like activated volume-regulated channels[3]. Trypan blue permits delineation of antimetabolite/tissue interactions without affecting cytoxicity for the assays investigated. Trypan blue can be used to visualise antimetabolite soaked sponges, estimate treatment area, and show areas of unintended tissue contact during trabeculectomy[4].

Diphenyl Blue 参考文献

[1]Sinumol S, Jayalakshmy PS, Sreedharan T, Unni MO, Narayan S, Sujatha N. Effect of intraoperative trypan blue on lens epithelial cells - Histomorphological analysis. Saudi J Ophthalmol. 2019 Jan-Mar;33(1):18-23

[2]Strober W. Trypan Blue Exclusion Test of Cell Viability. Curr Protoc Immunol. 2015 Nov 2;111:A3.B.1-A3.B.3

[3]Kerschbaum HH, Tasa BA, Schürz M, Oberascher K, Bresgen N. Trypan Blue - Adapting a Dye Used for Labelling Dead Cells to Visualize Pinocytosis in Viable Cells. Cell Physiol Biochem. 2021 Jun 23;55(S1):171-184

[4]Healey PR, Crowston JG. Trypan blue identifies antimetabolite treatment area in trabeculectomy. Br J Ophthalmol. 2005 Sep;89(9):1152-6

Diphenyl Blue 实验方案

计算器
存储液制备 1mg 5mg 10mg

1 mM

5 mM

10 mM

1.04mL

0.21mL

0.10mL

5.20mL

1.04mL

0.52mL

10.41mL

2.08mL

1.04mL

Diphenyl Blue 技术信息

CAS号72-57-1
分子式C34H24N6Na4O14S4
分子量 960.805
别名 曲利苯蓝 ;Direct Blue 14;Trypan Blue;NSC 11247;Visionblue
运输蓝冰
存储条件

液体 -20°C:3-6个月-80°C:12个月

粉末 Inert atmosphere,Room Temperature

溶解度

DMSO: 25 mg/mL(26.02 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

H2O: 1 mg/mL(1.04 mM),配合低频超声,并水浴加热至45℃助溶

动物实验配方
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