产品说明书
Diphenyl Blue
 |
同义名 : | 曲利苯蓝 ;Direct Blue 14;Trypan Blue;NSC 11247;Visionblue |
| CAS号 : | 72-57-1 | |
| 货号 : | A621935 | |
| 分子式 : | C34H24N6Na4O14S4 | |
| 纯度 : | 70% (dye content) | |
| 分子量 : | 960.805 | |
| MDL号 : | MFCD00003969 | |
| 存储条件： |
粉末 Inert atmosphere,Room Temperature 液体 -20°C:3-6个月-80°C:12个月 |
|
| 溶解度 : |
DMSO: 25 mg/mL(26.02 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO H2O: 1 mg/mL(1.04 mM)，配合低频超声，并水浴加热至45℃助溶 |
|
| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Trypan Blue is an acid azo dye commonly used as a stain to distinguish viable from non-viable cells. It is a vital stain used intra operatively during cataract surgery to stain the external surface of the anterior lens capsule for better visualization. Direct staining of the internal surface of anterior capsule with trypan blue affected LECs and the basement membrane. Treating the internal surface of capsular bag with trypan blue may reduce incidence of Posterior capsular opacification[1]. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells)[2]. In viable cells trypan blue-labelled vesicles indicate trypan blue uptake by macropinocytosis and trypan blue-labelled cytosol could indicate a further entry pathway for the dye, like activated volume-regulated channels[3]. Trypan blue permits delineation of antimetabolite/tissue interactions without affecting cytoxicity for the assays investigated. Trypan blue can be used to visualise antimetabolite soaked sponges, estimate treatment area, and show areas of unintended tissue contact during trabeculectomy[4]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
1.04mL 0.21mL 0.10mL |
5.20mL 1.04mL 0.52mL |
10.41mL 2.08mL 1.04mL |
| 参考文献 |
|---|