There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Coptisine chloride is an alkaloid from Chinese goldthread, and acts as an efficient uncompetitive IDO inhibitor with a Ki value of 5.8 μM and an IC50 value of 6.3 μM[1]. Coptisine (0.1-100 μM) inhibits the proliferation of A549, H460, H2170, MDA-MB-231 and HT-29 cells, with IC50s of 18.09, 29.50, 21.60, 20.15 and 26.60 µM, respectively. Coptisine (12.5, 25, 50 μM) upregulates the expression of pH2AX and p21, reduces expression of cyclin B1, cdc2, and cdc25C, and induces G2/M arrest and apoptosis of A549 cells in a concentration-dependent manner. Coptisine (12.5, 25, 50 μM) also induces mitochondrial dysfunction and activates caspases activities in A549 cells. Furthermore, Coptisine (50 μM) increases ROS levels in a time-dependent manner (0.5, 1, 2, 4, 12, and 24 h)[2]. Coptisine shows increased toxicity in mice in a concentration dependent manner, with LD50 value of 880.18 mg/kg. Coptisine (154 mg/kg/day, 90 days) shows no toxicity on SD rats. Coptisine (23.35, 46.7, 70.05 mg/kg, p.o.) dose-dependently decreases the levels of TC, TG, and LDL-c and increases HDL-c content in serum of hamsters to different degree, slows down weight gain induced by the HFHC (high-fat and high-cholesterol diet) diet, and raises the level of cholesterol and TBA (total bile acid) in feces dose-dependently in hamsters. Coptisine (70.05 mg/kg, p.o.) suppresses HMGCR (3-hydroxy-3-methyl-glutaryl-CoA reductase) protein expression level and induces the protein expression of SREBP-2, LDLR(low-density lipoprotein receptor), and CYP7A1 (cholesterol 7α-hydroxylase) involved in cholesterol metabolism[3].
Coptisine chloride/盐酸黄连碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
NCI-H1299
10 μM or 20 μM
24 h
To evaluate the effect of COP on ATF4 mRNA levels, results showed that COP significantly reduced ATF4 expression.
Adv Sci (Weinh). 2024 Sep;11(35):e2401748
NCI-H460
10 μM or 20 μM
24 h
To evaluate the effect of COP on ATF4 mRNA levels, results showed that COP significantly reduced ATF4 expression.
Adv Sci (Weinh). 2024 Sep;11(35):e2401748
Helicobacter pylori clinical isolates
25-50 μg/mL
3 days
To evaluate the antibacterial activity of Coptisine against clinical isolates, results showed that the MICs of Coptisine against clinical isolates were either 25 or 50 μg/mL
J Enzyme Inhib Med Chem. 2018 Dec;33(1):1362-1375
Helicobacter pylori standard strain NCTC 11637
25 μg/mL
3 days
To evaluate the antibacterial activity of Coptisine against H. pylori, results showed that the MIC of Coptisine against NCTC 11637 strain was 25 μg/mL
J Enzyme Inhib Med Chem. 2018 Dec;33(1):1362-1375
Hep3B cells
50 µM
24 h
To assess the cytotoxic effect of coptisine in Hep3B cells, results showed that coptisine significantly inhibited cell viability and induced apoptosis.
Int J Mol Sci. 2020 Jul 31;21(15):5502
MOLM-13 cells
0–20 μM
12 h
Evaluated the effect of Coptisine chloride on m6A modification levels, showing COP dose-dependently decreased m6A levels of mRNAs.
Adv Sci (Weinh). 2024 Jul;11(26):e2308786
A549 cells
10 μM
24 h
significantly lowered KRAS mRNA levels
Nat Commun. 2022 Oct 12;13(1):6016
H460 cells
10 μM
24 h
significantly lowered KRAS mRNA levels
Nat Commun. 2022 Oct 12;13(1):6016
Coptisine chloride/盐酸黄连碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
BALB/c nude mice
H460 xenograft tumor model
Intraperitoneal injection
100 mg/kg
Every other day for two weeks
To evaluate the effect of COP in glutamine restriction therapy, results showed that COP significantly enhanced the inhibitory effect of glutamine-restrictive therapy on tumor growth.
Adv Sci (Weinh). 2024 Sep;11(35):e2401748
Mice
Periodontitis model
Local gingival injection
20 μM
Daily until endpoint
Assessed the therapeutic effect of Coptisine chloride on periodontitis, showing COP significantly alleviated inflammatory bone loss and reduced IL-1β and GSDMD expression.
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