There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
The deacetylation of UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine (UDP-3-O-acyl-GlcNAc) by LpxC deacetylase is the committed reaction of lipid A biosynthesis. CHIR-090, a novel N-aroyl-l-threonine hydroxamic acid, is a competitive, two-step, slow, tight-binding inhibitor of Aquifex aeolicus LpxC. Similar to A. aeolicus LpxC, CHIR-090 inhibits E. coli LpxC by a two-step, time-dependent mechanism with a low nM Ki (1.0 - 1.7 nM for A. aeolicus LpxC versus 4.0 nM for E. coli LpxC). For LpxCs from other Gram-negative organisms, H. pylori LpxC, P. aeruginosa LpxC and N. meningitidis LpxC were inhibited 75 % or more by 4 nM CHIR-090 when enzyme was used to start the reactions. Given the relative resistance of W3110RL towards CHIR-090 (MIC of 100 μg/mL versus 0.25 μg/mL for W3110), conclusion can be made that CHIR-090 is indeed highly selective for LpxC in E. coli K-12[2].
作用机制
CHIR-090 contains a hydroxamate moiety coordinating the catalytic zinc ion. The binding of CHIR-090 is further strengthened through interactions with conserved hydrophilic and hydrophobic residues in the active site[1].
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