CHAPS is a non denaturing zwitterionic detergent for membrane biochemistry. It can be used to dissolve membrane proteins and interrupt protein-protein interactions. The zwitterionic detergent CHAPS, a derivative of the bile salts, is widely used in membrane protein solubilization. CHAPS has a low affinity for the erythrocyte membrane (partition coefficient K=0.06mM(-1)), and at sub-hemolytic concentrations it causes little effect on membrane lipid order. CHAPS hemolysis and phospholipid solubilization are closely correlated[1]. Chemical cross-linking shows that the dimerization of EnvZ-PD is significantly inhibited by the CHAPS and cholate[2]. The recovery and preservation of catalytic activity of two trans-membrane proteases, dipeptidyl peptidase IV and nephrilysin, was examined and found to be clearly superior after CHAPS treatment compared to DTT(dithiothreitol). Proteomic profiling by mass spectrometry (MS) revealed that 76.2% of proteins recovered by CHAPS are common to those seen for DTT treatment, which illustrates underlining similarities between the two approaches[3].
United States, California
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Beverly Hills Cancer Center
Recruiting
Beverly Hills, California, United States, 90211
Contact 310-432-8900
Principal Investigator: Linnea Chap 收起 <<
United States, District of Col... 展开 >>umbia
Childrens National Medical Center
Recruiting
Washington, District of Columbia, United States, 20010
Contact: Fahmida Hoq, MBBS, MS 202-476-3634 fhoq@cnmc.org 收起 <<
Australia, Queensland
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Queensland Centre for Intellectual and Developmental Disability, University of Queensland, Mater Hospital
Brisbane, Queensland, Australia, 4101 收起 <<
United States, California
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Premiere Oncology
Recruiting
Santa Monica, California, United States, 90404
Contact: Linnea I. Chap, M.D. 310-633-8400 lchap@premiereoncology.com
Contact: Marilyn Mulay, MSN (310) 633-8400 mmulay@premiereoncology.com
Principal Investigator: Linnea I. Chap, M.D. 收起 <<
United States, California
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VA Loma Linda Healthcare System, Loma Linda, CA
Loma Linda, California, United States, 92357
VA Long Beach Healthcare System, Long Beach, CA
Long Beach, California, United States, 90822
VA San Diego Healthcare System, San Diego, CA
San Diego, California, United States, 92161
VA Greater Los Angeles Healthcare System, West Los Angeles, CA
West Los Angeles, California, United States, 90073
United States, Nevada
VA Southern Nevada Healthcare System, North Las Vegas, NV
Las Vegas, Nevada, United States, 89106 收起 <<
Canada, Ontario
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Guelph-Wellington Emergency Medical Services and County of Wellington Social Services Department - Housing Services
Guelph, Ontario, Canada, N1H 3A1
Hamilton Paramedic Services
Hamilton, Ontario, Canada, L8P 4Y5
County of Simcoe
Simcoe, Ontario, Canada, L0L 1X0
City of Greater Sudbury
Sudbury, Ontario, Canada, P3A 5P3
York Region
York Region, Ontario, Canada, L3Y 6Z1 收起 <<
Diabetes Mellitus, Type 2
... 展开 >> Hypertension 收起 <<
Not Applicable
Recruiting
December 31, 2019
Philippines
... 展开 >> Ateneo de Zamboanga University
Recruiting
Zamboanga City, Zamboanga Del Sur, Philippines, 7000
Contact: Floro Dave Arnuco, MD 收起 <<
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