生物活性 | |||
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描述 | CHAPS is a non denaturing zwitterionic detergent for membrane biochemistry. It can be used to dissolve membrane proteins and interrupt protein-protein interactions. The zwitterionic detergent CHAPS, a derivative of the bile salts, is widely used in membrane protein solubilization. CHAPS has a low affinity for the erythrocyte membrane (partition coefficient K=0.06mM(-1)), and at sub-hemolytic concentrations it causes little effect on membrane lipid order. CHAPS hemolysis and phospholipid solubilization are closely correlated[1]. Chemical cross-linking shows that the dimerization of EnvZ-PD is significantly inhibited by the CHAPS and cholate[2]. The recovery and preservation of catalytic activity of two trans-membrane proteases, dipeptidyl peptidase IV and nephrilysin, was examined and found to be clearly superior after CHAPS treatment compared to DTT(dithiothreitol). Proteomic profiling by mass spectrometry (MS) revealed that 76.2% of proteins recovered by CHAPS are common to those seen for DTT treatment, which illustrates underlining similarities between the two approaches[3]. |
临床研究 | |||||
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NCT号 | 适应症或疾病 | 临床期 | 招募状态 | 预计完成时间 | 地点 |
NCT03241810 | Metastatic Breast Cancer | Phase 2 | Recruiting | September 2020 | - |
NCT03056755 | Breast Cancer | Phase 2 | Recruiting | April 10, 2020 | - |
NCT03155997 | Breast Cancer | Phase 3 | Recruiting | June 10, 2027 | - |
实验方案 | |||
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1mg | 5mg | 10mg | |
1 mM 5 mM 10 mM |
1.63mL 0.33mL 0.16mL |
8.13mL 1.63mL 0.81mL |
16.26mL 3.25mL 1.63mL |
参考文献 |
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