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描述 | Brilliant Blue G-250 is a dye widely used for protein visualization in SDS-PAGE, offering a straightforward staining procedure and effective quantitation. In the Bradford protein assay, protein concentrations are quantified by measuring the absorbance at 595 nm, which results from Brilliant Blue G-250 binding to proteins. Additionally, Brilliant Blue G-250 acts as a highly selective antagonist for the P2X7 receptor, effectively inactivating the NLRP3 inflammasome and showcasing its potential in therapeutic applications[1].[2].[3]. |
计算器 | ||||
存储液制备 | 1mg | 5mg | 10mg | |
1 mM 5 mM 10 mM |
1.17mL 0.23mL 0.12mL |
5.85mL 1.17mL 0.59mL |
11.71mL 2.34mL 1.17mL |
CAS号 | 6104-58-1 |
分子式 | C47H48N3NaO7S2 |
分子量 | 854.02 |
别名 | 酸性蓝 90 ;Brilliant Blue G;BBG;Acid blue 90, Coomassie Brilliant Blue G;NSC 328382;Coomassie Brilliant Blue G-250;CBBG;Acid Blue 90 |
运输 | 蓝冰 |
存储条件 |
粉末 Keep in dark place,Inert atmosphere,Room temperature 液体 -20°C:3-6个月-80°C:12个月 |
溶解度 |
DMSO: 12 mg/mL(14.05 mM),配合低频超声,并水浴加热至45℃助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO H2O: 10 mg/mL(11.71 mM),配合低频超声助溶 |
动物实验配方 |