产品说明书

Brilliant blue G-250

Print
Chemical Structure| 6104-58-1 同义名 : 酸性蓝 90 ;Brilliant Blue G;BBG;Acid blue 90, Coomassie Brilliant Blue G;NSC 328382;Coomassie Brilliant Blue G-250;CBBG;Acid Blue 90
CAS号 : 6104-58-1
货号 : A1372057
分子式 : C47H48N3NaO7S2
纯度 : BS
分子量 : 854.02
MDL号 : MFCD00078482
存储条件：

粉末 Keep in dark place,Inert atmosphere,Room temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 12 mg/mL(14.05 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 10 mg/mL(11.71 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 Brilliant Blue G-250 is a dye widely used for protein visualization in SDS-PAGE, offering a straightforward staining procedure and effective quantitation. In the Bradford protein assay, protein concentrations are quantified by measuring the absorbance at 595 nm, which results from Brilliant Blue G-250 binding to proteins. Additionally, Brilliant Blue G-250 acts as a highly selective antagonist for the P2X7 receptor, effectively inactivating the NLRP3 inflammasome and showcasing its potential in therapeutic applications[1].[2].[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.17mL

0.23mL

0.12mL

5.85mL

1.17mL

0.59mL

11.71mL

2.34mL

1.17mL
参考文献

[1]Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. 2009;(30):1431. Published 2009 Aug 3.

[2]Han XX, et al. Highly sensitive protein concentration assay over a wide range via surface-enhanced Raman scattering of Coomassie brilliant blue. Anal Chem. 2010;82(11):4325-4328.

[3]Zohny MH, et al. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need [published online ahead of print, 2022 Feb 21]. Biomed Pharmacother. 2022;148:112723.