2-NBDG | NBD-Glucose | Fluorescent Indicator | AmBeed-信号通路专用抑制剂

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2-(N-7-硝基-2,1,3-苯并噁二唑-4-氨基)-2-脱氧-D-葡萄糖 /2-NBDG {[allProObj[0].p_purity_real_show]}

货号：A422362 同义名： NBD-Glucose

2-NBDG is a fluorescent glucose analog for visualizing glucose uptake into living cells.

2-NBDG 化学结构
CAS号：186689-07-6

2-NBDG 3D分子结构
CAS号：186689-07-6

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2-NBDG 纯度/质量文件产品仅供科研

货号：A422362 标准纯度： {[allProObj[0].p_purity_real_show]} 产品说明书

批次查询：批次纯度: COA SDS NMR HPLC CNMR LC-MS

全球学术期刊中引用的产品: • JMC, 2024. Ambeed. [ A919389 , A918471 , A995972 ]; • Anal. Chem., 2024, 96(50): 19947-19954. Ambeed. [ A261947 , A1165388 ]; • ACS Biomater. Sci. Eng., 2024. Ambeed. [ A220462 ]; • ACS Biomater. Sci. Eng., 2024. Ambeed. [ A165235 , A110205 ]; • Pharmaceutics, 2024, 16(12): 1546. Ambeed. [ A272538 , A187261 , A691302 , A506702 ]; 更多 >

2-NBDG 生物活性

描述

2-NBDG, a fluorescent D-glucose analog, is a fluorescent indicator for monitoring glucose uptake into living cells. Ex: 467 nm; Em 542 nm. It has since been demonstrated in living mammalian cells that the uptake of 2-NBDG takes place through glucose transporters (GLUTs) in a concentration-, time- and temperature-dependent manner. A short-period application of 2-NBDG produced a remarkable increase in the fluorescence intensity in COS-1 cells over-expressing GLUT2, whereas the increase was barely detectable in mock-transfected cells. In mouse insulin-secreting clonal MIN6 cells, uptake was inhibited by cytochalasin B, a specific blocker for GLUTs, and by D-glucose in a dose-dependent manner^[1]. Circulating breast cancer cells with increased uptake of fluorescent 2-NBDG were detected in mice bearing human breast cancer xenograft tumors by fluorescence imaging, suggesting clinical use of 2-NBDG as a tracer for fluorescence imaging of hypermetabolic circulating breast cancer cells^[2]. 2-NBDG was continuously injected via the tail vein. Brain glucose metabolism was subsequently monitored by fluorescence imaging of 2-NBDG. The initial uptake rate of 2-NBDG at the injection site of 4-aminopyridine significantly exceeded that of the control injection site, which indicated local hypermetabolism induced by seizures. 2-NBDG can be used for localizing epileptic foci^[3].

2-NBDG 参考文献

[1]Zou C, Wang Y, Shen Z. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J Biochem Biophys Methods. 2005 Sep 30;64(3):207-15

[2]Cai H, Peng F. 2-NBDG fluorescence imaging of hypermetabolic circulating tumor cells in mouse xenograft model of breast cancer. J Fluoresc. 2013 Jan;23(1):213-20

[3]Tsytsarev V, Maslov KI, Yao J, Parameswar AR, Demchenko AV, Wang LV. In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog. J Neurosci Methods. 2012 Jan 15;203(1):136-40

2-NBDG 实验方案

计算器

存储液制备

1mg

5mg

10mg

1 mM

5 mM

10 mM

2.92mL

0.58mL

0.29mL

14.61mL

2.92mL

1.46mL

29.22mL

5.84mL

2.92mL

2-NBDG 技术信息

CAS号	186689-07-6
分子式	C₁₂H₁₄N₄O₈
分子量	342.262

别名	NBD-Glucose
运输	蓝冰

存储条件

In solvent -20°C:3-6个月-80°C:12个月

Pure form Inert atmosphere,Store in freezer, under -20°C

溶解方案

细胞实验：

H₂O: 5 mg/mL(14.61 mM)，配合低频超声，并水浴加热至45℃助溶

动物实验：配制的工作液建议现用现配，短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

动物实验配方

摩尔计算器

计算溶液所需的质量，体积或浓度。 Ambeed 摩尔浓度计算器基于以下方程式：

浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)=质量 (g)

浓度

体积

分子量

质量

2-(N-7-硝基-2,1,3-苯并噁二唑-4-氨基)-2-脱氧-D-葡萄糖 /2-NBDG {[allProObj[0].p_purity_real_show]}

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2-(N-7-硝基-2,1,3-苯并噁二唑-4-氨基)-2-脱氧-D-葡萄糖 /2-NBDG {[allProObj[0].p_purity_real_show]}

2-NBDG 纯度/质量文件 产品仅供科研

全球学术期刊中引用的产品

2-NBDG 质控信息

2-NBDG 生物活性

2-NBDG 参考文献

2-NBDG 实验方案

2-NBDG 技术信息

最近浏览的产品

大规格询价

摩尔计算器

靶点

总药量计算器

工作液计算器

细胞研究

临床研究

确认信息

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2-NBDG 纯度/质量文件产品仅供科研